• 产品名称Anti-PICK1抗体
    参阅全部 PICK1 一抗
  • 描述
    山羊多克隆抗体to PICK1
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Horse, Chicken, Cow, Dog, Human, Pig, Xenopus laevis, Chimpanzee
  • 免疫原

    Synthetic peptide:


    , corresponding to internal sequence amino acids 100-110 of Human PICK1 (NP_036539.1; NP_001034672.1; NP_001034673.1)

  • 阳性对照
    • Mouse and Rat brain lysates.



Our Abpromise guarantee covers the use of ab77178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 47 kDa.
ICC/IF Use at an assay dependent dilution.


  • 功能Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate heteromeric ACCN3/ACCN2 channel.
  • 组织特异性Ubiquitous.
  • 序列相似性Contains 1 AH domain.
    Contains 1 PDZ (DHR) domain.
  • 翻译后修饰Phosphorylation at Thr-82 appears to inhibit the interaction with AMPA receptors.
  • 细胞定位Cytoplasm > perinuclear region. Cell junction > synapse. Also present at excitatory synapses.
  • Information by UniProt
  • 数据库链接
  • 别名
    • dJ1039K5 antibody
    • MGC15204 antibody
    • OTTHUMP00000028509 antibody
    • PICK 1 antibody
    • Pick1 antibody
    • PICK1_HUMAN antibody
    • PRKCA binding protein antibody
    • PRKCA-binding protein antibody
    • PRKCABP antibody
    • Protein interacting with C kinase 1 antibody
    • Protein interacting with PRKCA antibody
    • Protein interacting with PRKCA 1 antibody
    • Protein kinase C alpha binding protein antibody
    • Protein kinase C-alpha-binding protein antibody
    • Protein that interacts with C kinase 1 antibody
    see all

Anti-PICK1 antibody 图像

  • Anti-PICK1 antibody (ab77178) at 1 µg/ml + mouse brain lysate in RIPA buffer at 35 µg

    Predicted band size : 47 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • ab77178 staining PICK1 in CHO cells stably transfected with Human PICK1 by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.5% Triton X-100 prior to blocking in 10% serum for 1 hour at room temperature. The primary antibody was diluted 1/1000 in PBS, 0.5% Triton, 10% FCS and incubated with the sample for 2 hours at °C. The secondary antibody was Alexa Fluor® 488-conjugated Donkey anti-Goat polyclonal, diluted 1/500.

    See Abreview

Anti-PICK1 antibody (ab77178)参考文献

ab77178 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Chinese Hamster Cultured Cells (CHO Cells stably transfected with human Pick1)
Specification CHO Cells stably transfected with human Pick1
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

提交于 May 03 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Stably transfected CHO cells expressing human Pick)
Loading amount 20 µg
Specification Stably transfected CHO cells expressing human Pick
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

提交于 Feb 22 2011