概述

  • 产品名称
  • 描述
    兔多克隆抗体to PICK1
  • 特异性
    Detects protein interacting with Protein Kinase C (PICK 1). By Western blot, this antibody detects an ~52 kDa protein representing PICK 1 from AtT20 cell extract. Immunocytochemical staining of CV1 cells transfected with PICK 1 results in a diffuse staining pattern when expressed alone and coclustered when coexpressed with mGluR7.
  • 经测试应用
    适用于: ICC, IP, WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Non human primates
    预测可用于: Cow, Cynomolgus monkey
  • 免疫原

    Synthetic peptide corresponding to Mouse PICK1 aa 1-18.
    Sequence:

    MFADLDYDIEEDKLGIPT


    (Peptide available as ab4964)

  • 阳性对照
    • WB: AtT20 cell extract ICC: CV1 cells transfected with PICK 1

性能

应用

Our Abpromise guarantee covers the use of ab3420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use a concentration of 1.7 µg/ml.
IP Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 52 kDa.Can be blocked with PICK1 peptide (ab4964).
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate heteromeric ACCN3/ACCN2 channel.
  • 组织特异性
    Ubiquitous.
  • 序列相似性
    Contains 1 AH domain.
    Contains 1 PDZ (DHR) domain.
  • 翻译后修饰
    Phosphorylation at Thr-82 appears to inhibit the interaction with AMPA receptors.
  • 细胞定位
    Cytoplasm > perinuclear region. Cell junction > synapse. Also present at excitatory synapses.
  • Information by UniProt
  • 数据库链接
  • 别名
    • dJ1039K5 antibody
    • MGC15204 antibody
    • OTTHUMP00000028509 antibody
    • PICK 1 antibody
    • Pick1 antibody
    • PICK1_HUMAN antibody
    • PRKCA binding protein antibody
    • PRKCA-binding protein antibody
    • PRKCABP antibody
    • Protein interacting with C kinase 1 antibody
    • Protein interacting with PRKCA antibody
    • Protein interacting with PRKCA 1 antibody
    • Protein kinase C alpha binding protein antibody
    • Protein kinase C-alpha-binding protein antibody
    • Protein that interacts with C kinase 1 antibody
    see all

Anti-PICK1 antibody 图像

  • ab3420 staining PICK1 in mouse Hippocampal neurons by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.1% saponin/PBS prior to blocking in 3% BSA for 1 hour at room temperature. The primary antibody was diluted 1/300 in 3% BSA, 0.1% saponin /PBS and incubated with the sample for 18 hours at 4 °C. The secondary antibody was Alexa Fluor® 455-conjugated Donkey anti-Rabbit polyclonal, diluted 1/1000.

  • Lane 1 : An myc antibody in PBST for 16 hours at 4°C
    Lane 2 : Anti-PICK1 antibody (ab3420) at 1/500 dilution (in PBST for 16 hours at 4°C)

    Lane 1 : Transfected CHO cells expressing human Pick1 tagged with myc
    Lane 2 : Transfected CHO cells expressing human Pick1 tagged with myc

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 2 : An HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : ~52 kDa (why is the actual band size different from the predicted?)
    Additional bands at : ~40 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

    This image is courtesy of an anonymous abreview.

    Blocking Step: 5% Milk for 1 hour at 20°C

    See Abreview

  • IHC image of ab3420 staining in rat adult brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3420, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab3420 staining PICK1 in CHO cells stably transfected with Human PICK1 by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.5% Triton X-100 prior to blocking in 10% serum for 1 hour at room temperature. The primary antibody was diluted 1/1000 in PBS, 0.5% Triton, 10% FCS and incubated with the sample for 2 hours at °C. The secondary antibody was Alexa Fluor® 488-conjugated Goat anti-Rabbit polyclonal, diluted 1/500.

    See Abreview

  • Western blot of PICK1 on AtT20 cell lysate using ab3420.

     

Anti-PICK1 antibody (ab3420)参考文献

This product has been referenced in:
  • Ma TM  et al. Pathogenic disruption of DISC1-serine racemase binding elicits schizophrenia-like behavior via D-serine depletion. Mol Psychiatry : (2012). Mouse . Read more (PubMed: 22801410) »
  • Thorsen TS  et al. Identification of a small-molecule inhibitor of the PICK1 PDZ domain that inhibits hippocampal LTP and LTD. Proc Natl Acad Sci U S A 107:413-8 (2010). IP ; Human . Read more (PubMed: 20018661) »

See all 2 Publications for this product

Product Wall

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Non-reduced Denaturing (8.5%)
Sample
Mouse Tissue lysate - whole (brain)
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Aug 01 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Sample
Mouse Cell (Hippocampal neurons)
Specification
Hippocampal neurons
Permeabilization
Yes - 0.1% saponin/PBS
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 01 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (primary hippocampal neuron, DIV14)
Specification
primary hippocampal neuron, DIV14
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton x-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Dec 17 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Chinese Hamster Cultured Cells (CHO Cells stably transfected with human Pick1)
Specification
CHO Cells stably transfected with human Pick1
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 May 03 2011

Application
Western blot
Sample
Chinese Hamster Cell lysate - whole cell (stably transfected CHO cells expressing human Pick)
Loading amount
20 µg
Specification
stably transfected CHO cells expressing human Pick
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Feb 14 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (INS1)
Loading amount
20 µg
Specification
INS1
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Jun 26 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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