Anti-Piccolo抗体[6H9-B6] (ab101654)

概述

  • 产品名称
    Anti-Piccolo抗体[6H9-B6]
    参阅全部 Piccolo 一抗
  • 描述
    小鼠单克隆抗体[6H9-B6] to Piccolo
  • 宿主
    Mouse
  • 特异性
    No cross reactivity to Bassoon.
  • 经测试应用
    适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Full length protein corresponding to Mouse Piccolo.
    Database link: Q9QYX7

  • 阳性对照
    • WB: Rat and mouse brain tissue lysate. ICC/IF: SK-N-BE cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 克隆编号
    6H9-B6
  • 同种型
    IgG2b
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab101654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 567 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF 1/100.

靶标

  • 功能
    May act as a scaffolding protein involved in the organization of synaptic active zones and in synaptic vesicle trafficking.
  • 序列相似性
    Contains 2 C2 domains.
    Contains 1 PDZ (DHR) domain.
  • 结构域
    C2 domain 1 is involved in binding calcium and phospholipids. Calcium binds with low affinity but with high specificity and induces a large conformational change.
  • 细胞定位
    Cell junction > synapse. Concentrated at the presynaptic side of synaptic junctions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACZ antibody
    • Aczonin antibody
    • Brain-derived HLMN protein antibody
    • DKFZp779G1236 antibody
    • KIAA0559 antibody
    • Multidomain presynaptic cytomatrix protein antibody
    • PCLO antibody
    • PCLO_HUMAN antibody
    • Piccolo (presynaptic cytomatrix protein) antibody
    • Piccolo, mouse, homolog of antibody
    • Pico antibody
    • Presynaptic cytomatrix protein antibody
    • Protein piccolo antibody
    see all

图片

  • SK-N-BE cells labeling Piccolo using ab101654 (green) at 1/100 dilution in ICC/IF. Cells were fixed using 4% formaldehyde for 15 minutes at room temperature. Primary antibody was incubated for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 at 1/100 dilution for 1 hour at room temperature. Counterstained with Phalloidin Texas Red F-actin stain. Nuclei were stained with DAPI (blue).

  • All lanes : Anti-Piccolo antibody [6H9-B6] (ab101654) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Predicted band size: 567 kDa

实验方案

文献

This product has been referenced in:
  • Mateos-Hernández L  et al. Quantitative proteomics reveals Piccolo as a candidate serological correlate of recovery from Guillain-Barré syndrome. Oncotarget 7:74582-74591 (2016). ELISA ; Human . Read more (PubMed: 27776345) »

See 1 Publication for this product

客户评价及客户问答

Thank you for your reply.

Yes, ab101654 is the only mous monoclonal antibody we have for Piccolo. It has not yet been tested with mouse samples and in IHC-P (paraffin-embedded PFA-fixed tissue sections). We cannot guarantee if and how well t...

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Vielen Dank für Ihren Anruf. Wie versprochen hatte ich für den anti-Piccolo- Antikörper ab101654 nachgefragt, ob das Epitop kartiert worden ist. Daraufhin hat man mir die Immunogensequenz geschickt, die zur Generierung dieses Antikörpers verwendet wurd...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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