Anti-Piccolo抗体[6H9-B6] (ab101654)
Key features and details
- Mouse monoclonal [6H9-B6] to Piccolo
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2b
概述
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产品名称
Anti-Piccolo抗体[6H9-B6]
参阅全部 Piccolo 一抗 -
描述
小鼠单克隆抗体[6H9-B6] to Piccolo -
宿主
Mouse -
特异性
No cross reactivity to Bassoon. -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Full length protein corresponding to Mouse Piccolo.
Database link: Q9QYX7 -
阳性对照
- WB: Rat and mouse brain tissue lysate. ICC/IF: SK-N-BE cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
6H9-B6 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab101654于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Predicted molecular weight: 567 kDa.
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|
ICC/IF |
1/100.
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说明 |
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WB
1/1000. Predicted molecular weight: 567 kDa. |
ICC/IF
1/100. |
靶标
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功能
May act as a scaffolding protein involved in the organization of synaptic active zones and in synaptic vesicle trafficking. -
序列相似性
Contains 2 C2 domains.
Contains 1 PDZ (DHR) domain. -
结构域
C2 domain 1 is involved in binding calcium and phospholipids. Calcium binds with low affinity but with high specificity and induces a large conformational change. -
细胞定位
Cell junction > synapse. Concentrated at the presynaptic side of synaptic junctions. - Information by UniProt
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数据库链接
- Entrez Gene: 27445 Human
- Entrez Gene: 26875 Mouse
- Entrez Gene: 56768 Rat
- Omim: 604918 Human
- SwissProt: Q9Y6V0 Human
- SwissProt: Q9QYX7 Mouse
- SwissProt: Q9JKS6 Rat
- Unigene: 12376 Human
see all -
别名
- ACZ antibody
- Aczonin antibody
- Brain-derived HLMN protein antibody
see all
图片
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SK-N-BE cells labeling Piccolo using ab101654 (green) at 1/100 dilution in ICC/IF. Cells were fixed using 4% formaldehyde for 15 minutes at room temperature. Primary antibody was incubated for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 at 1/100 dilution for 1 hour at room temperature. Counterstained with Phalloidin Texas Red F-actin stain. Nuclei were stained with DAPI (blue).
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All lanes : Anti-Piccolo antibody [6H9-B6] (ab101654) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Predicted band size: 567 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab101654 被引用在 1 文献中.
- Mateos-Hernández L et al. Quantitative proteomics reveals Piccolo as a candidate serological correlate of recovery from Guillain-Barré syndrome. Oncotarget 7:74582-74591 (2016). ELISA ; Human . PubMed: 27776345