Anti-PICALM抗体(ab113796)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to PICALM
  • 经测试应用
    适用于: IHC-Fr, WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    KLH conjugated synthetic peptide derived from the N terminal of Human PICALM.

  • 阳性对照
    • Human kidney tissue

性能

应用

Our Abpromise guarantee covers the use of ab113796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 71 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能
    Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction.
  • 组织特异性
    Expressed in all tissues examined.
  • 疾病相关
    Note=A chromosomal aberration involving PICALM is found in diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with MLLT10.
  • 序列相似性
    Contains 1 ENTH (epsin N-terminal homology) domain.
  • 细胞定位
    Membrane > clathrin-coated pit. Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle. Colocalized with clathrin in the Golgi area.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CALM antibody
    • Clathrin assembly lymphoid myeloid leukemia antibody
    • Clathrin assembly lymphoid myeloid leukemia protein antibody
    • CLTH antibody
    • LAP antibody
    • Phosphatidylinositol-binding clathrin assembly protein antibody
    • PICAL_HUMAN antibody
    • PICALM antibody
    see all

图片

  • ab113796 staining PICALM in Rat brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fresh-frozen, sliced to 18 microns, fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 2.5% serum for 60 minutes at 25°C. Samples were incubated with primary antibody (1/200 in PBS + 0.15% Triton + 1.5% normal Horse serum) for 20 hours at 25°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal (1/400) was used as the secondary antibody.

    See Abreview

  • ab113796, at 5 µg/ml, staining PICALM in formalin fixed, paraffin embedded Human kidney tissue by Immunohistochemistry

文献

ab113796 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (fresh-frozen rat brain sections, 18 micron)
Specification
fresh-frozen rat brain sections, 18 micron
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100 (0.3%)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
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提交于 Mar 28 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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