Anti-PICALM抗体(ab113796)

概述

  • 产品名称Anti-PICALM抗体
    参阅全部 PICALM 一抗
  • 描述
    兔多克隆抗体to PICALM
  • 经测试应用适用于: IHC-Fr, WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    KLH conjugated synthetic peptide derived from the N terminal of Human PICALM.

  • 阳性对照
    • Human kidney tissue

性能

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应用

Our Abpromise guarantee covers the use of ab113796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 71 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction.
  • 组织特异性Expressed in all tissues examined.
  • 疾病相关Note=A chromosomal aberration involving PICALM is found in diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with MLLT10.
  • 序列相似性Contains 1 ENTH (epsin N-terminal homology) domain.
  • 细胞定位Membrane > clathrin-coated pit. Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle. Colocalized with clathrin in the Golgi area.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CALM antibody
    • Clathrin assembly lymphoid myeloid leukemia antibody
    • Clathrin assembly lymphoid myeloid leukemia protein antibody
    • CLTH antibody
    • LAP antibody
    • Phosphatidylinositol-binding clathrin assembly protein antibody
    • PICAL_HUMAN antibody
    • PICALM antibody
    see all

Anti-PICALM antibody 图像

  • ab113796 staining PICALM in Rat brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fresh-frozen, sliced to 18 microns, fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 2.5% serum for 60 minutes at 25°C. Samples were incubated with primary antibody (1/200 in PBS + 0.15% Triton + 1.5% normal Horse serum) for 20 hours at 25°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal (1/400) was used as the secondary antibody.

    See Abreview

  • ab113796, at 5 µg/ml, staining PICALM in formalin fixed, paraffin embedded Human kidney tissue by Immunohistochemistry

Anti-PICALM antibody (ab113796)参考文献

ab113796 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (fresh-frozen rat brain sections, 18 micron)
Specification fresh-frozen rat brain sections, 18 micron
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100 (0.3%)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
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提交于 Mar 28 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"