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A 15 amino acid peptide from near the amino terminus of Human PICALM (NP_009097).
Our Abpromise guarantee covers the use of ab106409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 71 kDa.|
Immunofluorescence of PICALM in Human brain tissue with ab106409 at 20 ug/mL.
ICC/IF image of PICALM staining on blood smear using ab106409 (1:200). The blood film was fixed in paraformaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The cells were then blocked using 10% donkey serum for 1 hour at 24°C. ab106409 was diluted 1200 using and incubated with the cells for 4 hours at 24°C. The secondary antibody used was donkey polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000)
ab106409 has not yet been referenced specifically in any publications.