Anti-PIBF抗体(ab72118)
Key features and details
- Rabbit polyclonal to PIBF
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PIBF抗体
参阅全部 PIBF 一抗 -
描述
兔多克隆抗体to PIBF -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Rabbit, Horse, Dog, Rhesus monkey, Gorilla, Bat -
免疫原
Synthetic peptide within Human PIBF aa 735-757. The exact sequence is proprietary.
Database link: NP_006337.2 -
阳性对照
- WB: HeLa and 293T whole cell lysates. IP: HeLa whole cell lysate (ab150035).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab72118 was affinity purified using an epitope specific to PIBF immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72118于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Mediator of progesterone that by acting on the phospholipase A2 enzyme interferes with arachidonic acid metabolism, induces a Th2 biased immune response, and by controlling NK activity exerts an anti-abortive effect. -
组织特异性
Expressed at highest levels in testis. Moderate expression is detected in spleen, thymus, prostate, ovary, small intestine, and colon. - Information by UniProt
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数据库链接
- Entrez Gene: 10464 Human
- Omim: 607532 Human
- SwissProt: Q8WXW3 Human
- Unigene: 441926 Human
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别名
- C13orf24 antibody
- KIAA1008 antibody
- PIBF 1 antibody
see all
图片
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All lanes : Anti-PIBF antibody (ab72118) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 90 kDa
Observed band size: 90 kDa
Exposure time: 30 seconds -
Detection of PIBF by Western Blot of Immunprecipitate.
ab72118 at 1 µg/ml staining PIBF in HeLa whole cell lysate immunoprecipitated using ab72118 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab72118 被引用在 1 文献中.
- Wilsher S et al. The immunolocalization of Galectin-1 and Progesterone-Induced Blocking Factor (PIBF) in equine trophoblast: Possible roles in trophoblast invasion and the immunological protection of pregnancy. Placenta 85:32-39 (2019). PubMed: 31445347