概述

  • 产品名称Anti-PIAS3抗体
    参阅全部 PIAS3 一抗
  • 描述
    兔多克隆抗体to PIAS3
  • 特异性Detects endogenous levels of total PIAS3 protein
  • 经测试应用适用于: WB, IHC-P, ELISA, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from Internal of human PIAS3.

  • 阳性对照
    • Human lung carcinoma tissue 293 cells treated with UV (5 mins)

性能

应用

Our Abpromise guarantee covers the use of ab58406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.
IP Use at an assay dependent concentration.

靶标

  • 功能Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678, PubMed:9388184). Sumoylates CCAR2 which promotes its interaction with SIRT1 (PubMed:25406032). Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (PubMed:24651376).
  • 组织特异性Widely expressed.
  • 通路Protein modification; protein sumoylation.
  • 序列相似性Belongs to the PIAS family.
    Contains 1 PINIT domain.
    Contains 1 SAP domain.
    Contains 1 SP-RING-type zinc finger.
  • 结构域The PINIT domain of PIAS3 is required for STAT3-PIAS3 interaction and for transloaction to the nucleus.
    The LXXLL motif is a transcriptional coregulator signature.
  • 翻译后修饰Sumoylated.
  • 细胞定位Cytoplasm. Nucleus. Nucleus speckle. Colocalizes with MITF in the nucleus. Colocalizes with GFI1 in nuclear dots. Colocalizes with SUMO1 in nuclear granules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • E3 SUMO protein ligase PIAS 3 antibody
    • E3 SUMO protein ligase PIAS3 antibody
    • E3 SUMO-protein ligase PIAS3 antibody
    • FLJ14651 antibody
    • OTTHUMP00000015586 antibody
    • OTTHUMP00000015587 antibody
    • PIAS 3 antibody
    • Pias3 antibody
    • PIAS3 protein antibody
    • PIAS3_HUMAN antibody
    • Protein inhibitor of activated STAT 3 antibody
    • Protein inhibitor of activated STAT protein 3 antibody
    • Protein inhibitor of activated STAT3 antibody
    • Zinc finger MIZ type containing 5 antibody
    • ZMIZ 5 antibody
    • ZMIZ5 antibody
    see all

Anti-PIAS3 antibody 图像

  • Immunohistochemical analysis of paraffin embedded human lung carcinoma tissue using ab58406 at 1/50-1/100 dilution. Samples were treated -/+ peptide.
  • All lanes : Anti-PIAS3 antibody (ab58406) at 1/500 dilution

    Lane 1 : 293 cell extract treated with UV (5 min)
    Lane 2 : 293 cell extract treated with UV (5 min) with peptide


    Predicted band size : 67 kDa
    Observed band size : 67 kDa

Anti-PIAS3 antibody (ab58406)参考文献

This product has been referenced in:
  • Starsíchová A  et al. TGF-beta1 suppresses IL-6-induced STAT3 activation through regulation of Jak2 expression in prostate epithelial cells. Cell Signal 22:1734-44 (2010). WB ; Human . Read more (PubMed: 20603212) »

See 1 Publication for this product

Product Wall

Te agradezco mucho que nos hayáis enviado el cuestionario relleno, así como las imágenes. Revisando el protocolo todo parece correcto, y me temo que no tengo ninguna sugerencia que hacer para mejorarlo. Es posible que el lote concr...

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Application Immunoprecipitation
Sample Rat Cell lysate - whole cell (Rat Basophilic Leukemia)
Total protein in input 6e+006 cells
Specification Rat Basophilic Leukemia
Immuno-precipitation step Protein A/G
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提交于 Jun 30 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"