Anti-PIAS2抗体(ab99549)

概述

  • 产品名称Anti-PIAS2抗体
    参阅全部 PIAS2 一抗
  • 描述
    山羊多克隆抗体to PIAS2
  • 特异性This antibody is expected to recognize both reported isoforms (NP_775298.1; NP_004662.2).
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide:

    PQQVREICISRD

    , corresponding to internal sequence amino acids 185-196 of Human PIAS2 (NP_775298.1; NP_004662.2)

  • 阳性对照
    • Human Spleen and Peripheral Mononucleocytes lysates

性能

应用

Our Abpromise guarantee covers the use of ab99549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 60 kDa).

靶标

  • 功能Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'Lys-160'.
  • 组织特异性Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs.
  • 通路Protein modification; protein sumoylation.
  • 序列相似性Belongs to the PIAS family.
    Contains 1 PINIT domain.
    Contains 1 SAP domain.
    Contains 1 SP-RING-type zinc finger.
  • 发展阶段Isoform 3 expression in adult testis is 14.2-fold stronger than in embryonic testis.
  • 结构域The LXXLL motif is a transcriptional coregulator signature.
  • 翻译后修饰Sumoylated.
  • 细胞定位Nucleus speckle. Nucleus, PML body. Nucleus. Colocalizes at least partially with promyelocytic leukemia nuclear bodies (PML NBs) (PubMed:22406621). Colocalizes with SUMO1 in nuclear granules (By similarity).
  • Information by UniProt
  • 数据库链接
  • 别名
    • Androgen receptor interacting protein 3 antibody
    • Androgen receptor-interacting protein 3 antibody
    • ARIP3 antibody
    • DAB2 interacting protein antibody
    • DAB2-interacting protein antibody
    • DIP antibody
    • E3 SUMO protein ligase PIAS2 antibody
    • E3 SUMO-protein ligase PIAS2 antibody
    • MIZ antibody
    • Miz1 antibody
    • Msx interacting zinc finger protein antibody
    • Msx-interacting zinc finger protein antibody
    • PIAS NY protein antibody
    • PIAS-NY protein antibody
    • PIAS2 antibody
    • PIAS2_HUMAN antibody
    • PIASX antibody
    • PIASX-ALPHA antibody
    • PIASX-BETA antibody
    • Protein inhibitor of activated STAT x antibody
    • Protein inhibitor of activated STAT, 2 antibody
    • Protein inhibitor of activated STAT2 antibody
    • SIZ2 antibody
    • Zinc finger, MIZ-type containing 4 antibody
    • ZMIZ4 antibody
    see all

Anti-PIAS2 antibody 图像

  • Anti-PIAS2 antibody (ab99549) at 1 µg/ml + Human Peripheral Lymphocytes lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 60 kDa


    Exposure time : 1 hour

Anti-PIAS2 antibody (ab99549)参考文献

ab99549 has not yet been referenced specifically in any publications.

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