概述

  • 产品名称Anti-PIAS2抗体
    参阅全部 PIAS2 一抗
  • 描述
    小鼠多克隆抗体to PIAS2
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Full length Human PIAS2 (NP_775298.1).

  • 阳性对照
    • WB: Raw 264.7 and A431 cell lysates; PIAS2 transfected 293T cell lysates. ICC/IF: HeLa cells.

性能

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应用

Our Abpromise guarantee covers the use of ab88598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'Lys-160'.
  • 组织特异性Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs.
  • 通路Protein modification; protein sumoylation.
  • 序列相似性Belongs to the PIAS family.
    Contains 1 PINIT domain.
    Contains 1 SAP domain.
    Contains 1 SP-RING-type zinc finger.
  • 发展阶段Isoform 3 expression in adult testis is 14.2-fold stronger than in embryonic testis.
  • 结构域The LXXLL motif is a transcriptional coregulator signature.
  • 翻译后修饰Sumoylated.
  • 细胞定位Nucleus speckle. Nucleus, PML body. Nucleus. Colocalizes at least partially with promyelocytic leukemia nuclear bodies (PML NBs) (PubMed:22406621). Colocalizes with SUMO1 in nuclear granules (By similarity).
  • Information by UniProt
  • 数据库链接
  • 别名
    • Androgen receptor interacting protein 3 antibody
    • Androgen receptor-interacting protein 3 antibody
    • ARIP3 antibody
    • DAB2 interacting protein antibody
    • DAB2-interacting protein antibody
    • DIP antibody
    • E3 SUMO protein ligase PIAS2 antibody
    • E3 SUMO-protein ligase PIAS2 antibody
    • MIZ antibody
    • Miz1 antibody
    • Msx interacting zinc finger protein antibody
    • Msx-interacting zinc finger protein antibody
    • PIAS NY protein antibody
    • PIAS-NY protein antibody
    • PIAS2 antibody
    • PIAS2_HUMAN antibody
    • PIASX antibody
    • PIASX-ALPHA antibody
    • PIASX-BETA antibody
    • Protein inhibitor of activated STAT x antibody
    • Protein inhibitor of activated STAT, 2 antibody
    • Protein inhibitor of activated STAT2 antibody
    • SIZ2 antibody
    • Zinc finger, MIZ-type containing 4 antibody
    • ZMIZ4 antibody
    see all

Anti-PIAS2 antibody 图像

  • Anti-PIAS2 antibody (ab88598) at 1 µg/ml + Raw 264.7 cell lysate at 50 µg

    Predicted band size : 63 kDa
    Observed band size : 63 kDa
  • Anti-PIAS2 antibody (ab88598) at 1 µg/ml + A431 cell lysate at 50 µg

    Predicted band size : 63 kDa
    Observed band size : 63 kDa
  • All lanes : Anti-PIAS2 antibody (ab88598) at 1 µg/ml

    Lane 1 : PIAS2 transfected 293T cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 63 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • ab88598 at 10µg/ml staining PIAS2 in HeLa cells by Immunofluorescence.

Anti-PIAS2 antibody (ab88598)参考文献

ab88598 has not yet been referenced specifically in any publications.

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