重组
RabMAb

Anti-PIAS1抗体[EPR2580(2)] (ab109388)

概述

  • 产品名称
    Anti-PIAS1抗体[EPR2580(2)]
    参阅全部 PIAS1 一抗
  • 描述
    兔单克隆抗体[EPR2580(2)] to PIAS1
  • 经测试应用
    适用于: WB, IHC-P, ICC, Flow Cytmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PIAS1 aa 50-150. The exact sequence is proprietary.

  • 阳性对照
    • Human colon tissue. HepG2, Jurkat, 293T, Daudi cell lysates.
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab109388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 72 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
ICC 1/100 - 1/250.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明
    Is unsuitable for IP.
  • 靶标

    • 功能
      Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling.
    • 组织特异性
      Expressed in numerous tissues with highest level in testis.
    • 通路
      Protein modification; protein sumoylation.
    • 序列相似性
      Belongs to the PIAS family.
      Contains 1 PINIT domain.
      Contains 1 SAP domain.
      Contains 1 SP-RING-type zinc finger.
    • 结构域
      The LXXLL motif is a transcriptional coregulator signature.
      The SP-RING-type domain is required for promoting EKLF sumoylation.
    • 翻译后修饰
      Sumoylated.
      Dimethylated by PRMT1 at Arg-303 in the late phase of interferon gamma (IFN-gamma) signaling, leading to preferential interaction with STAT1 and thus resulting in release of STAT1 from its target gene.
    • 细胞定位
      Nucleus speckle. Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AR interacting protein antibody
      • DDXBP1 antibody
      • DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1 antibody
      • DEAD/H box binding protein 1 antibody
      • DEAD/H box-binding protein 1 antibody
      • E3 SUMO-protein ligase PIAS1 antibody
      • GBP antibody
      • Gu binding protein antibody
      • Gu-binding protein antibody
      • GU/RH-II antibody
      • Pias1 antibody
      • PIAS1_HUMAN antibody
      • Protein inhibitor of activated STAT protein 1 antibody
      • Protein inhibitor of activated STAT, 1 antibody
      • RNA helicase II binding protein antibody
      • RNA helicase II-binding protein antibody
      • Zinc finger, MIZ-type containing 3 antibody
      • ZMIZ3 antibody
      see all

    图片

    • Lane 1 : Anti-PIAS1 antibody [EPR2580(2)] (ab109388) at 1/500 dilution
      Lane 2 : Anti-GST antibody [EPR4236] (ab111947) at 1/2000 dilution

      Lane 1 : Recombinant Human PIAS1 protein (ab152888)
      Lane 2 : Recombinant Human PIAS1 protein (ab152888)

      Lysates/proteins at 0.01 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 72 kDa
      Observed band size : 98 kDa (why is the actual band size different from the predicted?)

      Exposure time:

      Lane 1: 10 seconds.

      Lane 2: 1 minute.

       

      Blocking and dilution buffer: 5% NFDM /TBST.

    • All lanes : Anti-PIAS1 antibody [EPR2580(2)] (ab109388) at 1/1000 dilution

      Lane 1 : Jurkat cell lysates
      Lane 2 : HepG2 cell lysates
      Lane 3 : 392T cell lysates
      Lane 4 : Daudi cell lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 72 kDa
    • Immunohistochemical analysis of paraffin-embedded Human colon tissue using 1/100 ab109388.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    文献

    This product has been referenced in:
    • Chanda A  et al. Identification of the SUMO E3 ligase PIAS1 as a potential survival biomarker in breast cancer. PLoS One 12:e0177639 (2017). WB, IF ; Human . Read more (PubMed: 28493978) »
    • Bogachek MV  et al. Inhibiting the SUMO Pathway Represses the Cancer Stem Cell Population in Breast and Colorectal Carcinomas. Stem Cell Reports 7:1140-1151 (2016). Read more (PubMed: 27916539) »

    See all 7 Publications for this product

    客户评价及客户问答

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton 5 minutes
    Specification
    HeLa
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Oct 26 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Mar 31 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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