概述

  • 产品名称Anti-PIAS1抗体
    参阅全部 PIAS1 一抗
  • 描述
    兔多克隆抗体to PIAS1
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from the internal sequence of Human PIAS1.

  • 阳性对照
    • MDA-MB-435 cell extract and Human lung carcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab58403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling.
  • 组织特异性Expressed in numerous tissues with highest level in testis.
  • 通路Protein modification; protein sumoylation.
  • 序列相似性Belongs to the PIAS family.
    Contains 1 PINIT domain.
    Contains 1 SAP domain.
    Contains 1 SP-RING-type zinc finger.
  • 结构域The LXXLL motif is a transcriptional coregulator signature.
    The SP-RING-type domain is required for promoting EKLF sumoylation.
  • 翻译后修饰Sumoylated.
    Dimethylated by PRMT1 at Arg-303 in the late phase of interferon gamma (IFN-gamma) signaling, leading to preferential interaction with STAT1 and thus resulting in release of STAT1 from its target gene.
  • 细胞定位Nucleus speckle. Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AR interacting protein antibody
    • DDXBP1 antibody
    • DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1 antibody
    • DEAD/H box binding protein 1 antibody
    • DEAD/H box-binding protein 1 antibody
    • E3 SUMO-protein ligase PIAS1 antibody
    • GBP antibody
    • Gu binding protein antibody
    • Gu-binding protein antibody
    • GU/RH-II antibody
    • Pias1 antibody
    • PIAS1_HUMAN antibody
    • Protein inhibitor of activated STAT protein 1 antibody
    • Protein inhibitor of activated STAT, 1 antibody
    • RNA helicase II binding protein antibody
    • RNA helicase II-binding protein antibody
    • Zinc finger, MIZ-type containing 3 antibody
    • ZMIZ3 antibody
    see all

Anti-PIAS1 antibody 图像

  • All lanes : Anti-PIAS1 antibody (ab58403) at 1/500 dilution

    Lane 1 : MDA-MB-435 cell extract with no immunizing peptide
    Lane 2 : MDA-MB-435 cell extract with immunizing peptide


    Predicted band size : 72 kDa
    Observed band size : 72 kDa
  • ab58403, at 1/50 dilution, staining human PIAS1 in lung carcinoma tissue, by immunohistochemistry, in the presence or absence of the immunizing peptide. Paraffin-embedded tissue.

Anti-PIAS1 antibody (ab58403)参考文献

This product has been referenced in:
  • Trzcinska-Daneluti AM  et al. High-content functional screen to identify proteins that correct F508del-CFTR function. Mol Cell Proteomics 8:780-90 (2009). WB ; Human . Read more (PubMed: 19088066) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"