重组
RabMAb

Anti-PIAS1 + PIAS2抗体[EPR2581Y] (ab77231)

概述

  • 产品名称Anti-PIAS1 + PIAS2抗体[EPR2581Y]
    参阅全部 PIAS1+PIAS2 一抗
  • 描述
    兔单克隆抗体[EPR2581Y] to PIAS1 + PIAS2
  • 经测试应用适用于: Flow Cyt, ICC/IF, WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    corresponding to Human PIAS1 aa 600 to the C-terminus (C terminal).
    Database link: O75925

  • 阳性对照
    • Daudi, 293, Jurkat, SW40 and HepG2 cell lysates; human tonsil tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab77231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/500.
WB 1/1000 - 1/10000. Predicted molecular weight: 72 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

靶标

Anti-PIAS1 + PIAS2 antibody [EPR2581Y] 图像

  • All lanes : Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231) at 1/50000 dilution

    Lane 1 : Human PIAS1 full-length recombinant protein with GST-Tag
    Lane 2 : Human PIAS2-beta recombinant protein fragment with GST-His-Tag
    Lane 3 : Human PIAS3 recombinant protein fragment with SUMO-His-Tag

    Lysates/proteins at 0.015 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 72 kDa
    Observed band size : kDa (why is the actual band size different from the predicted?)

    Blocking and Diluting buffer and concentration: 5% NFDM /TBST

    Exposure time: Lane1:10 seconds, Lane2: 5 seconds, Lane3: 3 minutes.

    Human PIAS1 full-length recombinant protein (Cat#: ab152888)

  • All lanes : Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231) at 1/50000 dilution

    Lane 1 : Daudi cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : SW40 cell lysate
    Lane 5 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 72 kDa
    Observed band size : 78 kDa (why is the actual band size different from the predicted?)
  • Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling PIAS1 + PIAS2 with purified ab77231 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

  • ab77231, at a 1/100 dilution, staining PIAS1 + PIAS2 in paraffin embedded human tonsil tissue by Immunohistochemistry.

Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231)参考文献

This product has been referenced in:
  • Malinen M  et al. Crosstalk between androgen and pro-inflammatory signaling remodels androgen receptor and NF-?B cistrome to reprogram the prostate cancer cell transcriptome. Nucleic Acids Res 45:619-630 (2017). WB, CHIPseq ; Human . Read more (PubMed: 27672034) »
  • Tao CC  et al. Epigenetic regulation of HDAC1 SUMOylation as an endogenous neuroprotection against Aß toxicity in a mouse model of Alzheimer's disease. Cell Death Differ 24:597-614 (2017). Read more (PubMed: 28186506) »

See all 7 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Hep3B)
Gel Running Conditions Non-reduced Denaturing
Loading amount 20 µg
Specification Hep3B
Blocking step I-Block(Applied biosystems) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 25°C
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提交于 Jun 19 2015

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (Hep3B)
Total protein in input 20 µg
Immuno-precipitation step Protein A
Specification Hep3B
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提交于 Jun 19 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (55.1C)
Permeabilization No
Specification 55.1C
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative Paraformaldehyde
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提交于 Jun 19 2015

Application ChIP
Sample Mouse Cell lysate - whole cell (primary hepatoctyes)
Negative control rabbit IgG
Specification primary hepatoctyes
Detection step Real-time PCR
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 8 minute(s) and 0 second(s)
Positive control H3K9ac
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提交于 Jun 19 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"