Anti-PI4K2A抗体- N-terminal (ab71824)

概述

  • 产品名称Anti-PI4K2A抗体- N-terminal
    参阅全部 PI4K2A 一抗
  • 描述
    兔多克隆抗体to PI4K2A - N-terminal
  • 经测试应用适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide from the N-terminal region of human PI4K2A conjugated to KLH

  • 阳性对照
    • HL60 cell lysate. Human hepatocarcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab71824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

靶标

  • 相关性Together with PI4K2B and the type III PI4Ks (PIK4CA and PIK4CB) PI4K2A contributes to the overall PI4 kinase activity of the cell. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells
  • 细胞定位Cytoplasm. Membrane. Note: Found in subdomains of the plasma membrane termed non caveolar membrane rafts.
  • 数据库链接
  • 别名
    • Phosphatidylinositol 4 kinase type 2 alpha antibody
    • Phosphatidylinositol 4 kinase type II alpha antibody
    • PI4KII antibody

Anti-PI4K2A antibody - N-terminal 图像

  • Anti-PI4K2A antibody - N-terminal (ab71824) at 1/100 dilution + HL60 cell lysate at 12.5 µg

    Secondary
    HRP-anti-rabbit

    Predicted band size : 54 kDa
    Observed band size : 54 kDa
  • ab71824 at 1/50 dilution, staining PI4K2A in human hepatocarcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-PI4K2A antibody - N-terminal (ab71824)参考文献

ab71824 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (NIH 3T3 mouse fibroblast cell line)
Loading amount 40 µg
Specification NIH 3T3 mouse fibroblast cell line
Treatment 48 hs-serum deprived
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. GERMAN GIL

Verified customer

提交于 Sep 22 2011

Thank you for your reply and further enquiry. I can confirm that only four nonconsecutive amino acids of the immunogenic sequence for ab71824 PI4K2A antibody are identical to PI4K2B. Although this has not been tested in the laboratory, it is unlike...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (multiple myeloma)
Total protein in input 300 µg
Specification multiple myeloma
Immuno-precipitation step Protein A
Username

Abcam user community

Verified customer

提交于 Jan 19 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"