概述

  • 产品名称Anti-PI3K p85抗体[M253]
    参阅全部 PI3K p85 一抗
  • 描述
    小鼠单克隆抗体[M253] to PI3K p85
  • 经测试应用适用于: WB, ICC, IHC-P, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken
  • 免疫原

    Recombinant fragment corresponding to the C-terminal region of Human PI3K p85

  • 阳性对照
    • A431 or Jurkat cell lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human skeletal muscle.
  • 常规说明

    Phosphoinositide 3-kinase (PI3K) phosphorylates phosphatidylinositol (PI), PI-4-phosphate and PI-4,5-bisphosphate to catalyze the production of PI-3,4,5-triphosphate. Growth factors and hormones activate PI3K to coordinate various cellular events, such as cell growth, cell cycle entry, cell migration and cell survival.

性能

应用

Our Abpromise guarantee covers the use of ab86714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
ICC 1/100.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use at an assay dependent concentration.
ELISA 1/2000.

Only tested in Peptide ELISA

靶标

  • 功能Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
  • 组织特异性Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).
  • 序列相似性Belongs to the PI3K p85 subunit family.
    Contains 1 Rho-GAP domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • 结构域The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.
  • 翻译后修饰Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
    Phosphorylated. Dephosphorylated by PTPRJ.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GRB 1 antibody
    • GRB1 antibody
    • p85 alpha antibody
    • p85 antibody
    • P85A_HUMAN antibody
    • Phosphatidylinositol 3 kinase associated p 85 alpha antibody
    • Phosphatidylinositol 3 kinase regulatory 1 antibody
    • Phosphatidylinositol 3 kinase regulatory subunit alpha antibody
    • Phosphatidylinositol 3 kinase regulatory subunit polypeptide 1 (p85 alpha) antibody
    • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody
    • Phosphatidylinositol 3-kinase regulatory subunit alpha antibody
    • Phosphoinositide 3 kinase regulatory subunit 1 (alpha) antibody
    • Phosphoinositide 3 kinase regulatory subunit 1 (p85 alpha) antibody
    • Phosphoinositide 3 kinase regulatory subunit 1 antibody
    • Phosphoinositide 3 kinase regulatory subunit polypeptide 1 (p85 alpha) antibody
    • PI3 kinase p85 subunit alpha antibody
    • PI3-kinase regulatory subunit alpha antibody
    • PI3-kinase subunit p85-alpha antibody
    • PI3K antibody
    • PI3K regulatory subunit alpha antibody
    • Pik3r1 antibody
    • PtdIns 3 kinase p85 alpha antibody
    • PtdIns-3-kinase regulatory subunit alpha antibody
    • PtdIns-3-kinase regulatory subunit p85-alpha antibody
    see all

Anti-PI3K p85 antibody [M253] 图像

  • Immunocytochemical labeling of PI3 Kinase p85 in aldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with ab86714, then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.

  • ICC/IF image of ab86714 stained SKNSH cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86714, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) (ab96871) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Lane 1 : Anti-PI3K p85 antibody [M253] (ab86714) at 1/1000 dilution
    Lane 2 : Anti-PI3K p85 antibody [M253] (ab86714) at 1/2000 dilution
    Lane 3 : Anti-PI3K p85 antibody [M253] (ab86714) at 1/4000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : A431 cell lysate


    Predicted band size : 85 kDa
    Observed band size : 85 kDa
  • IHC image of PI3K p85 staining in Human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (ab64236) (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86714, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-PI3K p85 antibody [M253] (ab86714)参考文献

This product has been referenced in:
  • Tantai JC  et al. Heterophyllin B inhibits the adhesion and invasion of ECA-109 human esophageal carcinoma cells by targeting PI3K/AKT/ß-catenin signaling. Mol Med Rep 13:1097-104 (2016). WB ; Human . Read more (PubMed: 26647768) »
  • Qian J  et al. Downregulation of HIF-1a inhibits the proliferation and invasion of non-small cell lung cancer NCI-H157 cells. Oncol Lett 11:1738-1744 (2016). Read more (PubMed: 26998070) »

See all 7 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (RWPE - prostate cells)
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Loading amount 30 µg
Specification RWPE - prostate cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

提交于 Oct 21 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (LNCaP - prostate cancer cells)
Permeabilization Yes - 0.2% Triton X-100
Specification LNCaP - prostate cancer cells
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 21 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Rat prostate)
Permeabilization Yes - 0.2% Triton X-100
Specification Rat prostate
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 19 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"