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Anti-PI3 Kinase p110 beta抗体[Y384] (ab32569)

概述

  • 产品名称Anti-PI3 Kinase p110 beta抗体[Y384]
    参阅全部 PI3 Kinase p110 beta 一抗
  • 描述
    兔单克隆抗体[Y384] to PI3 Kinase p110 beta
  • 特异性ab32569 recognises PI3-Kinases (PI3-Ks).
  • 经测试应用适用于: WB, Flow Cyt, IPmore details
    不适用于: ICC or IHC
  • 种属反应性
    与反应: Human
    预测可用于: Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    A synthetic peptide of human PI3-K p110 beta.

  • 阳性对照
    • Jurkat cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab32569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 110 kDa (predicted molecular weight: 123 kDa).
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.
  • 应用说明Is unsuitable for ICC or IHC.
  • 靶标

    • 功能Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
    • 组织特异性Expressed ubiquitously.
    • 通路Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
    • 序列相似性Belongs to the PI3/PI4-kinase family.
      Contains 1 PI3K/PI4K domain.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 5-bisphosphate 3-kinase 110 kDa catalytic subunit beta antibody
      • 5-bisphosphate 3-kinase catalytic subunit beta isoform antibody
      • DKFZp779K1237 antibody
      • MGC133043 antibody
      • OTTHUMP00000216901 antibody
      • OTTHUMP00000216904 antibody
      • p110 BETA antibody
      • p110Beta antibody
      • Phosphatidylinositol 3 kinase catalytic beta polypeptide antibody
      • Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit beta antibody
      • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit beta isoform antibody
      • Phosphatidylinositol-4 antibody
      • Phosphoinositide 3 kinase catalytic beta polypeptide antibody
      • PI3 kinase p110 subunit beta antibody
      • PI3-kinase subunit beta antibody
      • PI3K antibody
      • PI3K beta antibody
      • PI3K-beta antibody
      • PI3Kbeta antibody
      • PI3KCB antibody
      • PIK3C1 antibody
      • Pik3cb antibody
      • PK3CB_HUMAN antibody
      • PtdIns 3 kinase p110 antibody
      • PtdIns-3-kinase subunit beta antibody
      • PtdIns-3-kinase subunit p110-beta antibody
      see all

    Anti-PI3 Kinase p110 beta antibody [Y384] 图像



    • Predicted band size : 123 kDa

      Lane 1: Wild-type HAP1 cell lysate (40 µg)
      Lane 2: PI3 Kinase p110 beta knockout HAP1 cell lysate (40 µg)
      Lane 3: Jurkat cell lysate (40 µg)
      Lane 4: K562 cell lysate (40 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab32569 observed at 125 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab32569 was shown to recognize PI3 Kinase p110 beta when PI3 Kinase p110 beta knockout samples were used, along with additional cross-reactive bands. Wild-type and PI3 Kinase p110 beta knockout samples were subjected to SDS-PAGE. Ab32569 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) and IRDye® 680 Goat anti-Mouse IgG (H + L) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.

    • Anti-PI3 Kinase p110 beta antibody [Y384] (ab32569) at 1/500 dilution + Jurkat cell lysate

      Predicted band size : 123 kDa
      Observed band size : 110 kDa (why is the actual band size different from the predicted?)

    Anti-PI3 Kinase p110 beta antibody [Y384] (ab32569)参考文献

    This product has been referenced in:
    • Shugg RP  et al. Effects of isoform-selective phosphatidylinositol 3-kinase inhibitors on osteoclasts: actions on cytoskeletal organization, survival, and resorption. J Biol Chem 288:35346-57 (2013). WB ; Mouse . Read more (PubMed: 24133210) »
    • Conte E  et al. PI3K p110? overexpression in idiopathic pulmonary fibrosis lung tissue and fibroblast cells: in vitro effects of its inhibition. Lab Invest 93:566-76 (2013). WB ; Human . Read more (PubMed: 23439433) »

    See all 8 Publications for this product

    Product Wall

    Thank you for your enquiry regarding ab55593 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

    I would like to reassure you that our Abpromise ap...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (cancer cells)
    Loading amount 15 µg
    Specification cancer cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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    Abcam user community

    Verified customer

    提交于 Jun 07 2007

    Thank you for your enquiry. I am sorry but the sequence of the immunogen remains proprietary information. Rabbit monoclonal [Y384] to PI3 Kinase p110 beta (ab32569) has not been tested against the delta form of the protein. However, there appears t...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"