重组Anti-PI3 Kinase p110 beta抗体[EPR5515(2)] (ab151549)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5515(2)] to PI3 Kinase p110 beta
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-PI3 Kinase p110 beta抗体[EPR5515(2)]
参阅全部 PI3 Kinase p110 beta 一抗 -
描述
兔单克隆抗体[EPR5515(2)] to PI3 Kinase p110 beta -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IFmore details
不适用于: IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human PI3 Kinase p110 beta aa 400-500. The exact sequence is proprietary.
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阳性对照
- MCF-7, Jurkat, K562 and 293T cell lysates; Human brain tissue; 293 cells. Rat Brain Lysate, Mouse Brain Lysate
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5515(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Alexa Fluor® 488 Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab202666)
- Alexa Fluor® 647 Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab202668)
- Alexa Fluor® 594 Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab215509)
- Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] - BSA and Azide free (ab232690)
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab151549于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/20.
For unpurified use at 1/100 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB | (2) |
1/1000. Predicted molecular weight: 123 kDa.
For unpurified use at 1/1000 - 1/10000. |
ICC/IF |
1/150.
For unpurified use at 1/250 - 1/500. |
说明 |
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Flow Cyt (Intra)
1/20. For unpurified use at 1/100 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000. Predicted molecular weight: 123 kDa. For unpurified use at 1/1000 - 1/10000. |
ICC/IF
1/150. For unpurified use at 1/250 - 1/500. |
靶标
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功能
Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. -
组织特异性
Expressed ubiquitously. -
通路
Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis. -
序列相似性
Belongs to the PI3/PI4-kinase family.
Contains 1 PI3K/PI4K domain. - Information by UniProt
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数据库链接
- Entrez Gene: 5291 Human
- Entrez Gene: 74769 Mouse
- Entrez Gene: 85243 Rat
- Omim: 602925 Human
- SwissProt: P42338 Human
- SwissProt: Q3U4Q1 Mouse
- SwissProt: Q8BTI9 Mouse
- SwissProt: Q9Z1L0 Rat
see all -
别名
- 5-bisphosphate 3-kinase 110 kDa catalytic subunit beta antibody
- 5-bisphosphate 3-kinase catalytic subunit beta isoform antibody
- DKFZp779K1237 antibody
see all
图片
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All lanes : Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549) at 1/1000 dilution
Lane 1 : Rat Brain Lysate
Lane 2 : Mouse Brain Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution
Predicted band size: 123 kDa -
Overlay histogram showingSH-SY5Y cells stained with ab131260 (red line) at 1/20 dilution. The cells were fixed with 2% Paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (green line) was rabbit monoclonal IgG used under the same conditions.
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ab151549 staining PI3 Kinase p110 in the HeLa cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/150).ab150082 an Alexa Fluor®555-conjugated Goat anti-rabbit IgG(1/500) was used as the secondary antibody. Nuclei were counterstained with DAPI.
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Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549) at 1/5000 dilution + K562 Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution
Predicted band size: 123 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted? -
All lanes : Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549) at 1/1000 dilution
Lane 1 : 293 Cell Lysate
Lane 2 : Jurkat Cell Lysate
Lane 3 : MCF-7 Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution
Predicted band size: 123 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted? -
Overlay histogram showing HeLa cells stained with unpurified ab151549 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab151549, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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All lanes : Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549) at 1/1000 dilution (unpurified)
Lane 1 : MCF7 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 123 kDa -
Immunofluorescent analysis of 293 cells labelling PI3 Kinase p110 beta with unpurified ab151549 at 1/10 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (92)
ab151549 被引用在 92 文献中.
- Hou X et al. 3D-SeboSkin Model for Human ex vivo Studies of Hidradenitis Suppurativa/Acne Inversa. Dermatology 238:236-243 (2022). PubMed: 34077928
- Guo JQ et al. PDGF-BB/PDGFRβ promotes epithelial-mesenchymal transition by affecting PI3K/AKT/mTOR-driven aerobic glycolysis in Wilms' tumor G401 cells. Cell Biol Int 46:907-921 (2022). PubMed: 35165984
- Shi L et al. Cancer-associated fibroblast-derived exosomal microRNA-20a suppresses the PTEN/PI3K-AKT pathway to promote the progression and chemoresistance of non-small cell lung cancer. Clin Transl Med 12:e989 (2022). PubMed: 35857905
- Xu L et al. Treadmill exercise promotes E3 ubiquitin ligase to remove amyloid β and P-tau and improve cognitive ability in APP/PS1 transgenic mice. J Neuroinflammation 19:243 (2022). PubMed: 36195875
- Zhu M et al. Calsyntenin-1 Promotes Doxorubicin-induced Dilated Cardiomyopathy in Rats. Cardiovasc Drugs Ther N/A:N/A (2022). PubMed: 36350487