Anti-PI 4 Kinase type 2 beta抗体(ab37812)

概述

  • 产品名称Anti-PI 4 Kinase type 2 beta抗体
    参阅全部 PI 4 Kinase type 2 beta 一抗
  • 描述
    兔多克隆抗体to PI 4 Kinase type 2 beta
  • 特异性This antibody is specific for PI 4 Kinase type 2 beta.
  • 经测试应用适用于: ICC/IF, ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A KLH conjugated synthetic peptide selected from aa 1~17 of human PI 4 Kinase type 2 beta (N Terminal).

  • 阳性对照
    • HL-60 cell lysate and breast carcinoma tissue

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab37812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P 1/50 - 1/100.

靶标

  • 功能Together with PI4K2A and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. This contribution may be especially significant in plasma membrane, endosomal and Golgi compartments. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells and is likely to be involved in the regulation of vesicular trafficking.
  • 组织特异性Widely expressed.
  • 序列相似性Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.
    Contains 1 PI3K/PI4K domain.
  • 细胞定位Cytoplasm. Membrane. Mostly cytoplasmic but also found associated with the plasma membrane, the Golgi and endosomes. Compared to PI4K2A, a larger fraction of PI4K2B is cytosolic due to a smaller extent of palmitoylation. Translocates to membranes where it is recruited by PDGF stimulation by a Rac-GTP-dependent mechanism.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2610042N09Rik antibody
    • 4933409G22Rik antibody
    • FLJ11105 antibody
    • P4K2B_HUMAN antibody
    • Phosphatidylinositol 4 kinase type 2 beta antibody
    • Phosphatidylinositol 4 kinase type II beta antibody
    • Phosphatidylinositol 4-kinase type 2-beta antibody
    • Phosphatidylinositol 4-kinase type II-beta antibody
    • PI4K 2 beta antibody
    • PI4K 2b antibody
    • PI4K II beta antibody
    • PI4K IIb antibody
    • Pi4k2b antibody
    • PI4KII-BETA antibody
    • PI4KIIb antibody
    • PIK42B antibody
    • Type II phosphatidylinositol 4 kinase beta isoform antibody
    see all

Anti-PI 4 Kinase type 2 beta antibody 图像

  • Anti-PI 4 Kinase type 2 beta antibody (ab37812) at 1/100 dilution + HL-60 cell lysate

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
  • PI 4 Kinase type 2 beta expression detected on human breast cancer tissue using ab37812 at a 1/50 dilution (with a peroxidase-conjugated secondary antibody, followed by AEC staining).

    These data show the suitability of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

  • ICC/IF image of ab37812 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37812, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PI 4 Kinase type 2 beta antibody (ab37812)参考文献

ab37812 has not yet been referenced specifically in any publications.

Product Wall

Based on the very low homology (23%), ab37812 will very likely not detect mouse PI4K2B.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"