存放说明 Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
存储溶液 pH: 7.20 Preservative: 0.01% Sodium azide Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
纯度 Tissue culture supernatant
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 153 kDa).Can be blocked with
PI 3 Kinase regulatory subunit 4 peptide (ab183696).
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/100 - 1/500.
ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/100 - 1/250.
应用说明 Is unsuitable for IP.
功能 Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway.
组织特异性 Ubiquitously expressed.
序列相似性 Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 3 HEAT repeats. Contains 1 protein kinase domain. Contains 7 WD repeats.
翻译后修饰 Myristoylated. Probably autophosphorylated.
Information by UniProt
Phosphatidylinositol 3 kinase associated p150 antibody
Anti-PI 3 Kinase regulatory subunit 4 antibody [EPR5303(2)] 图像
Western blot - Anti-PI 3 Kinase regulatory subunit 4 antibody [EPR5303(2)] (ab124817)
All lanes : Anti-PI 3 Kinase regulatory subunit 4 antibody [EPR5303(2)] (ab124817) at 1/1000 dilution Lane 1 : Raji cell lysate Lane 2 : Jurkat cell lysate Lane 3 : Molt-4 cell lysate Lane 4 : 293T cell lysate Lane 5 : C6 cell lysate Lane 6 : Raw 264.7 cell lysate Lysates/proteins at 10 µg per lane. Secondary Goat-anti-rabbit HRP at 1/2000 dilution Predicted band size : 153 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase regulatory subunit 4 antibody [EPR5303(2)] (ab124817)
ab124817 at 1/100 dilution, staining PI 3 Kinase regulatory subunit 4 in Formalin fixed Paraffin-embedded Human kidney tissue by Immunohistochemistry.
Anti-PI 3 Kinase regulatory subunit 4 antibody [EPR5303(2)] (ab124817)参考文献
has not yet been referenced specifically in any publications.
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