Anti-PI 3 Kinase p85 beta抗体[T15] (ab28356)

概述

  • 产品名称
    Anti-PI 3 Kinase p85 beta抗体[T15]
    参阅全部 PI 3 Kinase p85 beta 一抗
  • 描述
    小鼠单克隆抗体[T15] to PI 3 Kinase p85 beta
  • 特异性
    T15 recognises the p85 beta subunit of bovine PI 3 kinase. It does not cross react with the p85 alpha subunit.
  • 经测试应用
    适用于: IHC-Fr, IP, WBmore details
  • 种属反应性
    与反应: Human, Monkey
    预测可用于: Mouse, Rat
  • 免疫原

    Balculovirus expressed (Recombinant) bovine P85 beta subunit of PI 3 kinase.

  • 阳性对照
    • Lysates of SF9 cells infected with baculovirus expressing p85 beta.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    This antibody is purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • 克隆
    单克隆
  • 克隆编号
    T15
  • 骨髓瘤
    NS1
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab28356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
IP 1/25. Use 25ul.
WB 1/1000 - 1/5000. Predicted molecular weight: 82 kDa.

靶标

  • 功能
    Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (PubMed:23604317). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement.
  • 疾病相关
    Megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 1
  • 序列相似性
    Belongs to the PI3K p85 subunit family.
    Contains 1 Rho-GAP domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • 结构域
    The SH2 2 domain is required for interaction with FBXL2 and PTPN13.
  • 翻译后修饰
    Phosphorylated in response to signaling from activated receptor-type protein kinases (PubMed:19690332, PubMed:20068231). Dephosphorylated by PTPRJ (PubMed:18348712). Dephosphorylated at Tyr-655 by PTPN13. Phosphorylation of Tyr-655 impairs while its dephosphorylation promotes interaction with FBXL2 and SCF(FBXL2)-mediated polyubiquitination (PubMed:23604317).
    Ubiquitinated. Polyubiquitination by the SCF(FBXL2) complex probably promotes proteasomal degradation of PIK3R2.
  • Information by UniProt
  • 数据库链接
  • 别名
    • p85 antibody
    • p85 beta antibody
    • P85B antibody
    • P85B_HUMAN antibody
    • Phosphatidylinositol 3 kinase antibody
    • Phosphatidylinositol 3 kinase regulatory beta subunit antibody
    • Phosphatidylinositol 3 kinase regulatory subunit beta antibody
    • Phosphatidylinositol 3 kinase regulatory subunit polypeptide 2 antibody
    • Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 2 (p85 beta) antibody
    • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta antibody
    • Phosphatidylinositol 3-kinase regulatory subunit beta antibody
    • Phosphoinositide 3 kinase regulatory subunit 2 (beta) antibody
    • Phosphoinositide 3 kinase regulatory subunit 2 antibody
    • Phosphoinositide 3 kinase regulatory subunit polypeptide 2 (p85 beta) antibody
    • Phosphoinositide 3 kinase regulatory subunit polypeptide 2 antibody
    • Phosphoinositide 3 kinase, regulatory subunit 2 (beta) antibody
    • Phosphoinositide 3 kinase, regulatory subunit 2 (p85 beta) antibody
    • PI3 kinase p85 beta subunit antibody
    • PI3 kinase p85 subunit beta antibody
    • PI3-kinase regulatory subunit beta antibody
    • PI3-kinase subunit p85-beta antibody
    • PI3K antibody
    • PI3K regulatory subunit beta antibody
    • PIK3R 2 antibody
    • PIK3R2 antibody
    • PtdIns 3 kinase p85 beta antibody
    • PtdIns-3-kinase regulatory subunit beta antibody
    • PtdIns-3-kinase regulatory subunit p85-beta antibody
    see all

图片



  • Predicted band size : 82 kDa

文献

This product has been referenced in:
  • Felli N  et al. AP2a controls the dynamic balance between miR-126&126* and miR-221&222 during melanoma progression. Oncogene 35:3016-26 (2016). WB . Read more (PubMed: 26434590) »
  • Du C  et al. MiR-126-3p suppresses tumor metastasis and angiogenesis of hepatocellular carcinoma by targeting LRP6 and PIK3R2. J Transl Med 12:259 (2014). Human . Read more (PubMed: 25240815) »

See all 5 Publications for this product

客户评价及客户问答

There are currently no Customer reviews or Questions for ab28356.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册