Anti-PI 3 Kinase p85 beta抗体(ab93778)

概述

  • 产品名称Anti-PI 3 Kinase p85 beta抗体
    参阅全部 PI 3 Kinase p85 beta 一抗
  • 描述
    兔多克隆抗体to PI 3 Kinase p85 beta
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide from a region within amino acids 75 - 125 of Human PI 3 Kinase p85 beta (NP_005018.1)

  • 阳性对照
    • HeLa cell lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab93778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 5-15µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 82 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (PubMed:23604317). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement.
    • 疾病相关Megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 1
    • 序列相似性Belongs to the PI3K p85 subunit family.
      Contains 1 Rho-GAP domain.
      Contains 2 SH2 domains.
      Contains 1 SH3 domain.
    • 结构域The SH2 2 domain is required for interaction with FBXL2 and PTPN13.
    • 翻译后修饰Phosphorylated in response to signaling from activated receptor-type protein kinases (PubMed:19690332, PubMed:20068231). Dephosphorylated by PTPRJ (PubMed:18348712). Dephosphorylated at Tyr-655 by PTPN13. Phosphorylation of Tyr-655 impairs while its dephosphorylation promotes interaction with FBXL2 and SCF(FBXL2)-mediated polyubiquitination (PubMed:23604317).
      Ubiquitinated. Polyubiquitination by the SCF(FBXL2) complex probably promotes proteasomal degradation of PIK3R2.
    • Information by UniProt
    • 数据库链接
    • 别名
      • p85 antibody
      • p85 beta antibody
      • P85B antibody
      • P85B_HUMAN antibody
      • Phosphatidylinositol 3 kinase antibody
      • Phosphatidylinositol 3 kinase regulatory beta subunit antibody
      • Phosphatidylinositol 3 kinase regulatory subunit beta antibody
      • Phosphatidylinositol 3 kinase regulatory subunit polypeptide 2 antibody
      • Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 2 (p85 beta) antibody
      • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta antibody
      • Phosphatidylinositol 3-kinase regulatory subunit beta antibody
      • Phosphoinositide 3 kinase regulatory subunit 2 (beta) antibody
      • Phosphoinositide 3 kinase regulatory subunit 2 antibody
      • Phosphoinositide 3 kinase regulatory subunit polypeptide 2 (p85 beta) antibody
      • Phosphoinositide 3 kinase regulatory subunit polypeptide 2 antibody
      • Phosphoinositide 3 kinase, regulatory subunit 2 (beta) antibody
      • Phosphoinositide 3 kinase, regulatory subunit 2 (p85 beta) antibody
      • PI3 kinase p85 beta subunit antibody
      • PI3 kinase p85 subunit beta antibody
      • PI3-kinase regulatory subunit beta antibody
      • PI3-kinase subunit p85-beta antibody
      • PI3K antibody
      • PI3K regulatory subunit beta antibody
      • PIK3R 2 antibody
      • PIK3R2 antibody
      • PtdIns 3 kinase p85 beta antibody
      • PtdIns-3-kinase regulatory subunit beta antibody
      • PtdIns-3-kinase regulatory subunit p85-beta antibody
      see all

    Anti-PI 3 Kinase p85 beta antibody 图像

    • All lanes : Anti-PI 3 Kinase p85 beta antibody (ab93778) at 0.04 µg/ml

      Lane 1 : Hela cell lysate at 50 µg
      Lane 2 : Hela cell lysate at 15 µg
      Lane 3 : HeLa cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 82 kDa


      Exposure time : 30 seconds
    • Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with antibody at 10 µg/mg lysate; 20% of immunoprecipitate loaded in each lane
      All lanes : PI 3 Kinase p85 beta antibody (ab93778) at 1 µg/ml
      Lane 1 : Lysate immunoprecipitated with ab93778
      Lane 2 : Lysate immunoprecipitated with a control IgG
      Predicted band size : 82 kDa
      Developed using the ECL technique
      Exposure time : 10 seconds

    Anti-PI 3 Kinase p85 beta antibody (ab93778)参考文献

    ab93778 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab93778.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"