Anti-PI 3 Kinase p85 beta抗体(ab93778)
Key features and details
- Rabbit polyclonal to PI 3 Kinase p85 beta
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PI 3 Kinase p85 beta抗体
参阅全部 PI 3 Kinase p85 beta 一抗 -
描述
兔多克隆抗体to PI 3 Kinase p85 beta -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide from a region within amino acids 75 - 125 of Human PI 3 Kinase p85 beta (NP_005018.1)
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阳性对照
- HeLa whole cell lysate (ab150035)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab93778于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 82 kDa.
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IP |
Use at 5-15 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 82 kDa. |
IP
Use at 5-15 µg/mg of lysate. |
靶标
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功能
Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (PubMed:23604317). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement. -
疾病相关
Megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 1 -
序列相似性
Belongs to the PI3K p85 subunit family.
Contains 1 Rho-GAP domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
结构域
The SH2 2 domain is required for interaction with FBXL2 and PTPN13. -
翻译后修饰
Phosphorylated in response to signaling from activated receptor-type protein kinases (PubMed:19690332, PubMed:20068231). Dephosphorylated by PTPRJ (PubMed:18348712). Dephosphorylated at Tyr-655 by PTPN13. Phosphorylation of Tyr-655 impairs while its dephosphorylation promotes interaction with FBXL2 and SCF(FBXL2)-mediated polyubiquitination (PubMed:23604317).
Ubiquitinated. Polyubiquitination by the SCF(FBXL2) complex probably promotes proteasomal degradation of PIK3R2. - Information by UniProt
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数据库链接
- Entrez Gene: 5296 Human
- Omim: 603157 Human
- SwissProt: O00459 Human
- Unigene: 371344 Human
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别名
- p85 antibody
- p85 beta antibody
- P85B antibody
see all
图片
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All lanes : Anti-PI 3 Kinase p85 beta antibody (ab93778) at 0.04 µg/ml
Lane 1 : Hela cell lysate at 50 µg
Lane 2 : Hela cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 82 kDa
Exposure time: 30 seconds -
Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with antibody at 10 µg/mg lysate; 20% of immunoprecipitate loaded in each lane
All lanes : PI 3 Kinase p85 beta antibody (ab93778) at 1 µg/ml
Lane 1 : Lysate immunoprecipitated with ab93778
Lane 2 : Lysate immunoprecipitated with a control IgG
Predicted band size : 82 kDa
Developed using the ECL technique
Exposure time : 10 seconds
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab93778 尚未被引用在任何文献中。