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A synthetic peptide corresponding to residues in Human PI3 Kinase p110 delta.
This product is a recombinant rabbit monoclonal antibody.
Alternative versions available:
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab109006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 119 kDa.|
|ICC||1/100 - 1/250.|
Lane 1: Wild type HAP1 whole cell lysate (40 µg)
Lane 2: PIK3CD knockout HAP1 whole cell lysate (40 µg)
Lane 3: Jurkat whole cell lysate (40 µg)
Lane 4: K562 whole cell lysate (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109006 observed at 120 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109006 was shown to specifically react with PIK3CD in wild-type HAP1 cells. No band was observed when PIK3CD knockout samples were used. Wild-type and PIK3CD knockout samples were subjected to SDS-PAGE. Ab109006 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.