兔多克隆抗体to PI 3 Kinase p110 delta
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 PI 3 Kinase p110 delta.
(Peptide available as
This antibody gave a positive signal in THP1 whole cell lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 119 kDa (predicted molecular weight: 119 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Expressed predominantly in leukocytes.
Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
Belongs to the PI3/PI4-kinase family.
Contains 1 PI3K/PI4K domain.
Autophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity.
Information by UniProt
5-bisphosphate 3-kinase 110 kDa catalytic subunit delta antibody
5-bisphosphate 3-kinase catalytic subunit delta isoform antibody
Western blot - PI 3 Kinase p110 delta antibody (ab82457)
Anti-PI 3 Kinase p110 delta antibody (ab82457) at 1 µg/ml + THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate at 10 µg
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution Developed using the ECL technique Performed under reducing conditions. Predicted band size : 119 kDa Observed band size : 119 kDa Additional bands at : 20 kDa,90 kDa. We are unsure as to the identity of these extra bands. Exposure time : 3 minutes
has not yet been referenced specifically in any publications.
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