Anti-PI 3 Kinase p110 delta抗体(ab1678)

概述

  • 产品名称
    Anti-PI 3 Kinase p110 delta抗体
    参阅全部 PI 3 Kinase p110 delta 一抗
  • 描述
    兔多克隆抗体to PI 3 Kinase p110 delta
  • 特异性
    Binds to a 110 Kd protein. Not likely to cross react with other isoforms due to sequence dissimilarity.
  • 经测试应用
    适用于: ICC/IF, IP, WB, ELISA, ICCmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    20 amino acid C-terminal peptide to Phosphoinositide 3 Kinase p110 delta

  • 阳性对照
    • ICC/IF: HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab1678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
IP Use at an assay dependent concentration. PubMed: 23023391
WB 1/2000 - 1/3000.
ELISA 1/75000.
ICC 1/400.

靶标

  • 组织特异性
    Expressed predominantly in leukocytes.
  • 通路
    Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
  • 序列相似性
    Belongs to the PI3/PI4-kinase family.
    Contains 1 PI3K/PI4K domain.
  • 翻译后修饰
    Autophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 5-bisphosphate 3-kinase 110 kDa catalytic subunit delta antibody
    • 5-bisphosphate 3-kinase catalytic subunit delta isoform antibody
    • APDS antibody
    • GRB1 antibody
    • IMD14 antibody
    • p110d antibody
    • p110delta antibody
    • p110dp85a antibody
    • p85-ALPHA antibody
    • Phosphatidylinositol 3 kinase catalytic delta polypeptide antibody
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit delta isoform antibody
    • Phosphatidylinositol 4,5 bisphosphate 3 kinase 110 kDa catalytic subunit delta antibody
    • Phosphatidylinositol 4,5 bisphosphate 3 kinase, catalytic subunit delta antibody
    • Phosphatidylinositol 45 bisphosphate 3 kinase catalytic subunit delta isoform antibody
    • Phosphatidylinositol-4 antibody
    • Phosphoinositide 3 kinase B antibody
    • Phosphoinositide 3 kinase C antibody
    • Phosphoinositide 3 kinase catalytic delta polypeptide antibody
    • Phosphoinositide 3 kinase, catalytic, delta polypeptide variant p37delta antibody
    • PI3 kinase p110 subunit delta antibody
    • PI3-kinase subunit delta antibody
    • PI3K antibody
    • PI3K-delta antibody
    • PI3Kdelta antibody
    • Pik3cd antibody
    • PIK3R1 antibody
    • PK3CD antibody
    • PK3CD_HUMAN antibody
    • PtdIns 3 kinase p110 antibody
    • PtdIns 3 kinase subunit p110 delta antibody
    • PtdIns-3-kinase subunit delta antibody
    • PtdIns-3-kinase subunit p110-delta antibody
    see all

图片

  • All lanes : Anti-PI 3 Kinase p110 delta antibody (ab1678) at 1/2000 dilution

    Lane 1 : Peripheral Blood Mononuclear Cells (PBMCs)
    Lane 2 : Primary human monocytes
    Lane 3 : Primary human macropahges
    Lane 4 : U937 monocytic cell line
    Lane 5 : Whole human Lung

    Lysates/proteins at 20 µg per lane.

    Secondary
    Bovine anti-Rabbit IgG-HRP at 1/2000 dilution
    Developed using the ECL technique

    Exposure time : 1 minute

    Abreview from Verifed customer

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  • ICC/IF image of ab1678 stained HeLa (human epithelial cell line from cervix adenocarcinoma) cells. The cells were 4% formaldehyde fixed (10 minutes) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1678, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.

文献

This product has been referenced in:
  • Yea SS  et al. Effects of novel isoform-selective phosphoinositide 3-kinase inhibitors on natural killer cell function. PLoS One 9:e99486 (2014). WB . Read more (PubMed: 24915189) »
  • Suárez-Fueyo A  et al. Inhibition of PI3Kd reduces kidney infiltration by macrophages and ameliorates systemic lupus in the mouse. J Immunol 193:544-54 (2014). Mouse . Read more (PubMed: 24935930) »

See all 10 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (K652 cells)
Loading amount
5000 cells
Specification
K652 cells
Treatment
DMSO or 1 uM CAL-101 for 24 hours
Gel Running Conditions
Reduced Denaturing (4-20% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Mar 21 2013

Thank you for your enquiry regarding ab55593 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

I would like to reassure you that our Abpromise ap...

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Thank you for your data and for bringing this article to our attention.

The immunogen sequence is

*************

Is that included in the p37delta sequence?

I am glad to see the antibody was specific for the p1...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (monocyte, macrophage, whole lung)
Loading amount
20 µg
Specification
monocyte, macrophage, whole lung
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Sep 03 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (cancer cells)
Loading amount
15 µg
Specification
cancer cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Jun 07 2007

We have no evidence that PI3Kinase ab1678 is a blocking antibody

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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