RabMAb

Anti-PI 3 Kinase Class 3抗体[EPR5301] (ab124905)

概述

  • 产品名称Anti-PI 3 Kinase Class 3抗体[EPR5301]
    参阅全部 PI 3 Kinase Class 3 一抗
  • 描述
    兔单克隆抗体[EPR5301] to PI 3 Kinase Class 3
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • 阳性对照
    • L6, C2C12, 293T, SHYSY-5Y cell lysates; Mouse brain tissue lysate; Human gastric adenocarcinoma tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab124905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 102 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for Flow Cyt,ICC or IP.
  • 靶标

    • 功能Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis.
    • 组织特异性Ubiquitously expressed, with a highest expression in skeletal muscle.
    • 序列相似性Belongs to the PI3/PI4-kinase family.
      Contains 1 PI3K/PI4K domain.
    • 细胞定位Midbody.
    • Information by UniProt
    • 数据库链接
    • 别名
      • hVps34 antibody
      • MGC61518 antibody
      • Phosphatidylinositol 3 kinase catalytic subunit type 3 antibody
      • Phosphatidylinositol 3 kinase class 3 antibody
      • Phosphatidylinositol 3 kinase p100 subunit antibody
      • Phosphatidylinositol 3-kinase catalytic subunit type 3 antibody
      • Phosphatidylinositol 3-kinase p100 subunit antibody
      • Phosphoinositide 3 kinase class 3 antibody
      • Phosphoinositide-3-kinase class 3 antibody
      • PI3 kinase type 3 antibody
      • PI3-kinase type 3 antibody
      • PI3K type 3 antibody
      • Pik3c3 antibody
      • PK3C3_HUMAN antibody
      • PtdIns 3 kinase type 3 antibody
      • PtdIns-3-kinase type 3 antibody
      • Vps 34 antibody
      • Vps34 antibody
      see all

    Anti-PI 3 Kinase Class 3 antibody [EPR5301] 图像

    • All lanes : Anti-PI 3 Kinase Class 3 antibody [EPR5301] (ab124905) at 1/1000 dilution

      Lane 1 : L6 cell lysate
      Lane 2 : C2C12 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : Mouse brain tissue lysate
      Lane 5 : SHSY-5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 102 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    • ab124905, at 1/50 dilution, staining PI 3 Kinase in paraffin-embedded Human gastric adenocarcinoma tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-PI 3 Kinase Class 3 antibody [EPR5301] (ab124905)参考文献

    This product has been referenced in:
    • Bechtel W  et al. Vps34 deficiency reveals the importance of endocytosis for podocyte homeostasis. J Am Soc Nephrol 24:727-43 (2013). Read more (PubMed: 23492732) »

    See 1 Publication for this product

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