概述

  • 产品名称
    Anti-PI 3 Kinase Class 3抗体
    参阅全部 PI 3 Kinase Class 3 一抗
  • 描述
    兔多克隆抗体to PI 3 Kinase Class 3
  • 经测试应用
    适用于: IHC-P, ICC/IF, ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to amino acids 39-54 of Human PI3KC 3.

  • 阳性对照
    • HeLa cell lysate.
  • 常规说明


    Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • Primary antibody说明
    Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100.
ICC/IF Use a concentration of 5 µg/ml.
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 101 kDa (predicted molecular weight: 106 kDa).

靶标

  • 功能
    Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis.
  • 组织特异性
    Ubiquitously expressed, with a highest expression in skeletal muscle.
  • 序列相似性
    Belongs to the PI3/PI4-kinase family.
    Contains 1 PI3K/PI4K domain.
  • 细胞定位
    Midbody.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hVps34 antibody
    • MGC61518 antibody
    • Phosphatidylinositol 3 kinase catalytic subunit type 3 antibody
    • Phosphatidylinositol 3 kinase class 3 antibody
    • Phosphatidylinositol 3 kinase p100 subunit antibody
    • Phosphatidylinositol 3-kinase catalytic subunit type 3 antibody
    • Phosphatidylinositol 3-kinase p100 subunit antibody
    • Phosphoinositide 3 kinase class 3 antibody
    • Phosphoinositide-3-kinase class 3 antibody
    • PI3 kinase type 3 antibody
    • PI3-kinase type 3 antibody
    • PI3K type 3 antibody
    • Pik3c3 antibody
    • PK3C3_HUMAN antibody
    • PtdIns 3 kinase type 3 antibody
    • PtdIns-3-kinase type 3 antibody
    • Vps 34 antibody
    • Vps34 antibody
    see all

Anti-PI 3 Kinase Class 3 antibody 图像



  • Predicted band size : 106 kDa


    ab5451 at a 1/100 dilution staining approximately 101kDa band of PI3KC 3 in HeLa cell lysate by Western blot (ECL).
  • ab5451 staining PI 3 Kinase Class 3 in human hepatocarcinoma (HC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ICC/IF image of ab5451 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5451, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PI 3 Kinase Class 3 antibody (ab5451)参考文献

This product has been referenced in:
  • Zhou J  et al. Saxifragifolin D attenuates phagosome maturation arrest in Mycobacterium tuberculosis-infected macrophages via an AMPK and VPS34-dependent pathway. AMB Express 7:11 (2017). Read more (PubMed: 28050854) »
  • Han J  et al. A Complex between Atg7 and Caspase-9: A NOVEL MECHANISM OF CROSS-REGULATION BETWEEN AUTOPHAGY AND APOPTOSIS. J Biol Chem 289:6485-97 (2014). Read more (PubMed: 24362031) »

See all 5 Publications for this product

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