RabMAb

Anti-PI 3 Kinase catalytic subunit gamma抗体[Y388] (ab32089)

概述

  • 产品名称Anti-PI 3 Kinase catalytic subunit gamma抗体[Y388]
    参阅全部 PI 3 Kinase catalytic subunit gamma 一抗
  • 描述
    兔单克隆抗体[Y388] to PI 3 Kinase catalytic subunit gamma
  • 特异性This antibody is specific for PI 3 Kinase catalytic subunit gamma.
  • 经测试应用适用于: WB, Flow Cyt, IPmore details
    不适用于: ICC or IHC
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues in the N terminal of Human PI 3 Kinase catalytic subunit gamma.

  • 表位ab32089 reacts with an epitope located in the N terminal region of PI 3 Kinase catalytic subunit gamma
  • 阳性对照
    • Jurkat cell lysate.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab32089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 126 kDa.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.
  • 应用说明Is unsuitable for ICC or IHC.
  • 靶标

    • 功能3-phosphorylates the cellular phosphoinositide PtdIns-4,5-biphosphate (PtdIns(4,5)P2) to produce PtdIns-3, 4,5-triiphosphate (PtdIns(3,4,5)P3). Links G-protein coupled receptor activation to the secondary messenger PtdIns(3,4,5)P3 production.
    • 组织特异性Pancreas, skeletal muscle, liver and heart.
    • 通路Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
    • 序列相似性Belongs to the PI3/PI4-kinase family.
      Contains 1 PI3K/PI4K domain.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 1 phosphatidylinositol 3 kinase antibody
      • 5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma antibody
      • 5-bisphosphate 3-kinase catalytic subunit gamma isoform antibody
      • p110 gamma antibody
      • p110gamma antibody
      • p120 PI3K antibody
      • p120-PI3K antibody
      • Phosphatidylinositol 3 kinase catalytic 110 kD gamma antibody
      • Phosphatidylinositol 3 kinase gamma, p110 gamma antibody
      • Phosphatidylinositol 3 kinase, catalytic, gamma polypeptide antibody
      • Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit gamma antibody
      • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit gamma antibody
      • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit gamma isoform antibody
      • Phosphatidylinositol-4 antibody
      • Phosphoinositide 3 kinase catalytic gamma polypeptide antibody
      • Phosphoinositide 3 kinase gamma catalytic subunit antibody
      • PI3 kinase p110 subunit gamma antibody
      • PI3-kinase subunit gamma antibody
      • PI3CG antibody
      • PI3K antibody
      • PI3K-gamma antibody
      • PI3Kgamma antibody
      • PIK3 antibody
      • Pik3cg antibody
      • PK3CG_HUMAN antibody
      • PtdIns-3-kinase subunit gamma antibody
      • PtdIns-3-kinase subunit p110-gamma antibody
      • Serine/threonine protein kinase PIK3CG antibody
      see all

    Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388] 图像

    • Overlay histogram showing HeLa cells stained with ab32089 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32089, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388] (ab32089) at 1/1000 dilution + Jurkat cell lysate.

      Predicted band size : 126 kDa
      Observed band size : 110 kDa (why is the actual band size different from the predicted?)

    Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388] (ab32089)参考文献

    This product has been referenced in:
    • Conte E  et al. PI3K p110? overexpression in idiopathic pulmonary fibrosis lung tissue and fibroblast cells: in vitro effects of its inhibition. Lab Invest 93:566-76 (2013). Human . Read more (PubMed: 23439433) »
    • Conte E  et al. Inhibition of PI3K prevents the proliferation and differentiation of human lung fibroblasts into myofibroblasts: the role of class I P110 isoforms. PLoS One 6:e24663 (2011). WB ; Human . Read more (PubMed: 21984893) »

    See all 2 Publications for this product

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