The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Detects a band of approximately 126 kDa.
1/10 - 1/50.
3-phosphorylates the cellular phosphoinositide PtdIns-4,5-biphosphate (PtdIns(4,5)P2) to produce PtdIns-3, 4,5-triiphosphate (PtdIns(3,4,5)P3). Links G-protein coupled receptor activation to the secondary messenger PtdIns(3,4,5)P3 production.
ab70912, at a 1/10 dilution, staining human PI 3 Kinase catalytic subunit gamma in muscle, using Immunohistochemistry, Formalin/PFA fixed Paraffin embedded tissue. ab70912 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Conte E et al. Inhibition of PI3K Prevents the Proliferation and Differentiation of Human Lung Fibroblasts into Myofibroblasts: The Role of Class I P110 Isoforms. PLoS One6:e24663 (2011).
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