Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 199-228 of Human Phostensin.
Ramos cell lysate; Human spleen tissue
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
ab103327 is purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100 - 1/500. Predicted molecular weight: 68 kDa.
Protein phosphatase-1 (PP1) interacts with regulatory subunits that target the enzyme to different cellular locations and change its activity toward specific substrates. Phostensin is a regulatory subunit that targets PP1 to F-actin cytoskeleton.
Western blot - Phostensin antibody (ab103327)
Anti-Phostensin antibody (ab103327) at 1/100 dilution + Ramos cell lysate at 35 µg
Predicted band size : 68 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Phostensin antibody (ab103327)
ab103327, at 1/50 dilution, staining Phostensin in formalin-fixed, paraffin-embedded Human spleen tissue by Immunohistochemistry using a peroxidase conjugated secondary antibody and DAB staining.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"