Phosphotyrosine In-Cell ELISA试剂盒(ab126431)

概述

  • 产品名称
    Phosphotyrosine In-Cell ELISA试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Adherent cells
  • 检测类型
    Cell-based (qualitative)
  • 检测时间
    5h 10m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 产品概述

    ab126431 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of Phosphotyrosine and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human, mouse and rat cell lines. By determining Phosphotyrosine protein in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort in preparing cell lysate and performing an analysis of Western Blot.

    In the Cell-Based Phosphotyrosine ELISA kit, cells are seeded into a 96 well tissue culture plate. The cells are fixed after various treatments, inhibitors or activators. After blocking, HRP-Anti- Phosphotyrosine is pipetted into the wells and incubated. The wells are washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of protein. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用
    适用于: In-Cell ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab126431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
In-Cell ELISA Use at an assay dependent concentration.

图片

  • A431 cells were treated for 30 min with 50 µL of 100 mM Genistein or 2 mM Lavendustin in appropriate wells at room temperature prior to EGF stimulation. Added 50 µL different concentrations of rhEGF (0, 20 or 100 ng/ml in serum free DMEM) to appropriate wells. Then incubated for 10 min at 37°C.

  • A431 cells were treated for 30 min with 50 µl of 100 mM Genistein or 2 mM Lavendustin in appropriate wells at room temperature prior to EGF stimulation. Added 50 µl different concentrations of rhEGF (0, 20 or 100 ng/ml in serum free DMEM) to appropriate wells. Then incubated for 10 min at 37°C.

实验方案

文献

ab126431 has not yet been referenced specifically in any publications.

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