Anti-Phosphotyrosine抗体[PY20] (Rhodamine) (ab28346)

概述

  • 产品名称Anti-Phosphotyrosine抗体[PY20] (Rhodamine)
    参阅全部 Phosphotyrosine 一抗
  • 描述
    小鼠单克隆抗体[PY20] to Phosphotyrosine (Rhodamine)
  • 偶联物Rhodamine. Ex: 550nm, Em: 570nm
  • 特异性Ab28346 specifically recognizes phosphorylated tyrosine residues and does not react with phosphorylated threonine or serine residues.
  • 经测试应用适用于: ICC, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Species independent
  • 免疫原

    Phosphotyrosine coupled to carrier protein.

  • 常规说明

    Ab28346 is especially useful for the detection of these phosphorylated tyrosine proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest. The antibody is known to be inhibited by divalent cations (>1mM) and high salt concentrations (>0.2M).

    The purified antibody was labeled by reaction with tetramethyl rhodamine (TAMRA) succinimidyl ester in the presence of phenyl phosphate (to protect the antibody binding site). The unreacted TAMRA succinimidyl ester was removed by molecular exclusion chromatography.

性能

应用

Our Abpromise guarantee covers the use of ab28346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use a concentration of 5 - 10 µg/ml.
IHC-Fr Use a concentration of 5 - 10 µg/ml.
WB 1/5000.

靶标

  • 相关性The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

Anti-Phosphotyrosine antibody [PY20] (Rhodamine) (ab28346)参考文献

ab28346 has not yet been referenced specifically in any publications.

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