Anti-Phosphotyrosine抗体[PY20] (Biotin) (ab16388)

概述

  • 产品名称Anti-Phosphotyrosine抗体[PY20] (Biotin)
    参阅全部 Phosphotyrosine 一抗
  • 描述
    小鼠单克隆抗体[PY20] to Phosphotyrosine (Biotin)
  • 偶联物Biotin
  • 特异性Clone PY20 specifically recognizes phosphorylated tyrosine residues and does not react with phosphorylated threonine or serine residues.
  • 经测试应用适用于: WB, ELISA, ICCmore details
  • 种属反应性
    与反应: Species independent
  • 免疫原

    Phosphotyrosine coupled to carrier protein

  • 常规说明

    This antibody is known to be inhibited by divalent cations (>1mM) and high salt concentrations (>0.2M).

    The purified antibody is coupled through free primary amino groups to biotin. The binding site of the antibody was protected by the addition of phenyl phosphate prior to coupling.

性能

应用

Our Abpromise guarantee covers the use of ab16388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
ICC
  • 应用说明
    ELISA: 1/5000.
    ICC: 1/500.
    WB: 1/5000.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

    Anti-Phosphotyrosine antibody [PY20] (Biotin) (ab16388)参考文献

    ab16388 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application ELISA
    Sample Human Recombinant protein (YSPTSPS (CTD repeat) peptide)
    Specification YSPTSPS (CTD repeat) peptide
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 22°C
    Type Direct
    Username

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    Verified customer

    提交于 Mar 14 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"