Anti-Phosphotyrosine抗体[IG2] (ab5592)

概述

  • 产品名称
    Anti-Phosphotyrosine抗体[IG2]
    参阅全部 Phosphotyrosine 一抗
  • 描述
    小鼠单克隆抗体[IG2] to Phosphotyrosine
  • 特异性
    ab5592 detects phosphotyrosine from most species and tissues. ab5592 will not react with any other phosphorylated amino acids, proteins or nucleotides.
  • 经测试应用
    适用于: ELISA, ICC/IF, WB, ICC, Flow Cyt, IPmore details
  • 种属反应性
    与反应: Species independent
  • 免疫原

    Chemical/ Small Molecule corresponding to Phosphotyrosine. O-phospho-L-tyrosine and O-phospho-DL-tyramine.

  • 阳性对照
    • HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab5592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
ICC/IF 1/50 - 1/200.
WB 1/100 - 1/1000.
ICC 1/50 - 1/200.
Flow Cyt Use at an assay dependent concentration.
IP Use a concentration of 2 µg/ml.

靶标

  • 相关性
    The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

图片

  • Immunolocalization of phosphotyrosine in HeLa cells using ab5592.

文献

ab5592 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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