生物素Anti-Phosphotyrosine抗体(ab9331)
Key features and details
- Biotin Rabbit polyclonal to Phosphotyrosine
- Suitable for: WB, IP, ELISA
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG
概述
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产品名称
生物素Anti-Phosphotyrosine抗体
参阅全部 Phosphotyrosine 一抗 -
描述
生物素兔多克隆抗体to Phosphotyrosine -
宿主
Rabbit -
偶联物
Biotin -
特异性
Recognizes both free phosphotyrosine (pY) and tyrosine-phosphorylated peptide and proteins. -
经测试应用
适用于: WB, IP, ELISAmore details -
种属反应性
与反应: Species independent -
免疫原
Chemical/ Small Molecule corresponding to Phosphotyrosine conjugated to keyhole limpet haemocyanin.
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阳性对照
- Use Vanadium treated cell culture (30 min) for Western Blotting and Tyrosine-phosphorylated peptide for ELISA.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 6
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Immunoaffinity chromatography with phosphotyrosine-agarose. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Isotype control
应用
应用 | Ab评论 | 说明 |
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WB | ||
IP | ||
ELISA |
说明 |
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IP: Use at 16µg/mg of lysate. Acetone precipitation of the protein extract followed by SDS denaturation is recommended.
WB: 1/125. Not recommended for WB of trace cellular phospho-protein. Acetone precipitation of the protein extract followed by SDS denaturation is recommended.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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相关性
The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
图片
数据表及文件
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Datasheet download
文献 (0)
ab9331 尚未被引用在任何文献中。