Anti-Phosphotyrosine抗体(Biotin) (ab9331)

概述

  • 产品名称
    Anti-Phosphotyrosine抗体(Biotin)
    参阅全部 Phosphotyrosine 一抗
  • 描述
    兔多克隆抗体to Phosphotyrosine (Biotin)
  • 偶联物
    Biotin
  • 特异性
    Recognizes both free phosphotyrosine (pY) and tyrosine-phosphorylated peptide and proteins.
  • 经测试应用
    适用于: WB, IP, ELISAmore details
  • 种属反应性
    与反应: Species independent
  • 免疫原

    Chemical/ Small Molecule conjugated to KLH.

  • 阳性对照
    • Use Vanadium treated cell culture (30 min) for Western Blotting and Tyrosine-phosphorylated peptide for ELISA.

性能

应用

Our Abpromise guarantee covers the use of ab9331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
ELISA
  • 应用说明
    ELISA: 1/500.
    IP: Use at 16µg/mg of lysate. Acetone precipitation of the protein extract followed by SDS denaturation is recommended.
    WB: 1/125. Not recommended for WB of trace cellular phospho-protein. Acetone precipitation of the protein extract followed by SDS denaturation is recommended.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

    图片

    • Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B)

    文献

    ab9331 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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