Anti-Phosphotyrosine抗体(ab50722)

概述

  • 产品名称Anti-Phosphotyrosine抗体
    参阅全部 Phosphotyrosine 一抗
  • 描述
    兔多克隆抗体to Phosphotyrosine
  • 特异性ab50722 recognizes tyrosine phosphorylated proteins. The antibody recognizes the pY-256 random peptide and phospho-L-tyrosine, but does not recognize phospho-L-serine, phospho-Lthreonine or L-tyrosine.
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Species independent
  • 免疫原

    Phospho-L-tyrosine containing random peptide pY-256, conjugated to KLH.

  • 阳性对照
    • Total cell extract of A431 stimulated by human EGF
  • 常规说明


    Antibodies specific for phosphotyrosine are essential tools for the characterization of tyrosine phosphorylation in many signal transduction pathways. Polyclonal antibodies can offer advantages over monoclonals, especially in immunoprecipitation protocols, and can theoretically react with a broader range of phosphotyrosine-containing proteins.

性能

应用

Our Abpromise guarantee covers the use of ab50722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 10 kDa.
ICC/IF Use a concentration of 5 - 10 µg/ml.

靶标

  • 相关性The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

Anti-Phosphotyrosine antibody 图像

  • All lanes : Anti-Phosphotyrosine antibody (ab50722) at 0.3 µg/ml

    Lane 1 : Whole cell extracts of untreated A431 cells
    Lane 2 : Whole cell extracts of EGF-treated A431 cells
    Lane 3 : Whole cell extracts of peroxyvanadate-treated A431 cells

    Secondary
    Goat Anti-Rabbit IgG-Peroxidase conjugate using a chemiluminescent substrate
    Developed using the ECL technique

    Predicted band size : 10 kDa
  • Plasma membrane and cytoplasmic staining A431 Cells were fixed with paraformaldheyde and stained with ab50722 at 1/100, followed by Goat anti-Rabbit IgG (H+L), FITC conjugate. Panel A: Serum-starved A431 cells stimulated with human EGF (1 µg/mL, 5 min, EGF human). Panel B: Untreated control A431 cells.

Anti-Phosphotyrosine antibody (ab50722)参考文献

ab50722 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"