Anti-Phosphotyrosine抗体(ab17302)

概述

  • 产品名称
    Anti-Phosphotyrosine抗体
    参阅全部 Phosphotyrosine 一抗
  • 描述
    兔多克隆抗体to Phosphotyrosine
  • 特异性
    Minimal to no crossreactivity with phosphothreonine or phosphoserine containing proteins.
  • 经测试应用
    适用于: ELISA, WB, IPmore details
  • 种属反应性
    与反应: Species independent
  • 免疫原

    Affinity purified native tyrosine phosphorylated proteins (Human).

  • 阳性对照
    • A431 Cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab17302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000 - 1/10000.
WB 1/1000 - 1/2000.
IP Use at an assay dependent dilution. Use 2.5 µg pwe IP reaction.

靶标

  • 相关性
    The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

图片

  • All lanes : Anti-Phosphotyrosine antibody (ab17302)

    Lane 1 : Untreated parotid acinar cells
    Lane 2 : Parotid acinar cells with 50µM etoposide for 2 hours
    Lane 3 : Parotid acinar cells with 5mM H2O2 for 30 min

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP-conjugated Donkey anti-Rabbit IgG at 1/10000 dilution

    Performed under reducing conditions.

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview.

    See Abreview

文献

ab17302 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Parotid acinar cells)
Loading amount
50 µg
Specification
Parotid acinar cells
Treatment
untreated (UT), + 50uM etoposide for 2 hours (E), + 5mM H2O2 for 30 min (H)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Nov 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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