Anti-Phosphothreonine-X-Phosphotyrosine抗体(HRP) (ab9342)

概述

  • 产品名称Anti-Phosphothreonine-X-Phosphotyrosine抗体(HRP)
    参阅全部 Phosphothreonine-X-Phosphotyrosine 一抗
  • 描述
    兔多克隆抗体to Phosphothreonine-X-Phosphotyrosine (HRP)
  • 偶联物HRP
  • 特异性ELISA test indicated that this antibody specifically reacted to peptide containing pT-G-pY, pT-E-pY sequence, but weakly reacted to pT-P-pY sequence. The antibody does not react with phosphothreonine and phosphoserine but reacts weakly with phosphotyrosine.
  • 经测试应用适用于: WB, IP, ELISAmore details
  • 种属反应性
    与反应: Species independent
  • 免疫原

    KLH-phosphothreonine-lysine-phosphotyrosine (N-acetyl-pT-K-pY-NH2) peptide conjugate.

  • 阳性对照
    • Mouse brain extract for immunoblotting. Synthetic pT-X-pY containing phosphopeptide for ELISA.
  • 常规说明

    Antigen is based on N-acetyl-pT-K-pY-NH2. The pT-X-pY sequence is considered to be the motif for some active MAPK's.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Phosphate-buffered Saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Immunoaffinity chromatography with N-acetyl-phosphothreonine-lysine-phosphotyrosine amide agarose, absorbed with N-acetyl-threonine-lysine-tyrosine agarose.
  • Primary antibody说明Antigen is based on N-acetyl-pT-K-pY-NH2. The pT-X-pY sequence is considered to be the motif for some active MAPK's
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250.
IP Use at an assay dependent concentration.
ELISA 1/500.

Anti-Phosphothreonine-X-Phosphotyrosine antibody (HRP) 图像

  • Immunoblotting of a human fibroblast cell lysate (4 hour UV treated and non-treated) with anti-pT-X-pY antibodies.
  • Immunoblotting of protein extracts with anti-pT-X-pY antibodies

    A: Extract from Mouse Brain
    B: Extract from Mouse Brain teated with Okadaic Acid(0.1 uM, 30 min).
    C: Lysate of Melanoma Cell
    D: Lysate of Melanoma Cell treated with Okadaic Acid.

Anti-Phosphothreonine-X-Phosphotyrosine antibody (HRP) (ab9342)参考文献

ab9342 has not yet been referenced specifically in any publications.

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