• 产品名称Anti-Phosphothreonine抗体
    参阅全部 Phosphothreonine 一抗
  • 描述
    兔多克隆抗体to Phosphothreonine
  • 特异性Reacts with phosphorylated threonine both as free amino acid or when conjugated to a carrier protein such as BSA. Also, cross reacts with phosphorylated tyrosine.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Species independent
  • 免疫原

    Phosphothreonine conjugated to KLH.



Our Abpromise guarantee covers the use of ab6187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明ELISA: Use at an assay dependant dilution.
    WB: 1/200 - 1/2000.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation. Protein phosphorylation and dephosphorylation are basic mechanisms for the modification of protein function in eukaryotic cells. Phosphorylation is a rare post-translational event in normal tissue, however, the abundance of phosphorylated cellular proteins increases several fold following various activation processes which are mediated through phosphotyrosine, phosphoserine or phosphothreonine (p-tyr/p-ser/p-thr). Many signal transduction pathways, such as the EGF, PDGF and insulin receptor systems, contain tyr/ser/thr kinase which phosphorylate specific tyr/ser/thr residues upon binding of ligands to their receptors. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate these phosphorylation associated kinases, and cells transformed by viral oncogenes contain elevated levels of phosphorylated tyr/ser/thr. An understanding of transformation by oncogenes and mitogenic processes of growth factors depends on the identification of their substrate and a subsequent determination of how phosphorylation affects their properties. Studies on the role of phosphorylated proteins have been hampered by their low abundance and the problem of distinguishing the various types of phosphorylated proteins. The most common procedure is to label intact cells or small tissue fragments with 32P and subsequently to isolate 32P labeled proteins by conventional biochemical methods. In order to identify the specific amino acids that undergo phosphorylation, additional long and tedious procedures for phosphoamino acid analysis are required. Immunoblotting of cellular proteins with antibodies directed against phosphoamino acids is advantageous as it does not involve 32P labeling, and can therefore be employed to monitor alterations in phosphorylation of specific proteins as they occur in intact organs or the whole animal. Indeed, mono and polyclonal antibodies directed against phosphorylated residues have been generated and found useful as analytical and preparative tools because they enable the rapid identification, quantification and immunoaffinity isolation of phosphorylated cellular proteins.

    Anti-Phosphothreonine antibody 图像

    Anti-Phosphothreonine antibody (ab6187)参考文献

    ab6187 has not yet been referenced specifically in any publications.

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    We only have an ELISA protocol for this antibody. We suggest that you follow standard protocols for blotting and SDS-PAGE.