Anti-Phospho - (Ser/Thr)抗体(ab117253)
Key features and details
- Rabbit polyclonal to Phospho - (Ser/Thr)
- Suitable for: ELISA, Dot blot
- Reacts with: Species independent
- Isotype: IgG
概述
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产品名称
Anti-Phospho - (Ser/Thr)抗体 -
描述
兔多克隆抗体to Phospho - (Ser/Thr) -
宿主
Rabbit -
经测试应用
适用于: ELISA, Dot blotmore details -
种属反应性
与反应: Species independent -
免疫原
Phosphoserine/threonine conjugated with R-PE.
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常规说明
Buffers and proteins which contain phosphate should be avoided with this antibody. Certain proteins known to contain phosphorylated serine and threonine may not be detected by this antibody due to steric hindrance.
The immunogen used is Phosphoserine and phosphothreonine conjugated with R-PE.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.16% Tris HCl, 0.88% Sodium chloride -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab117253于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ELISA |
1/10000 - 1/50000.
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|
Dot blot |
Use at an assay dependent dilution.
|
说明 |
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ELISA
1/10000 - 1/50000. |
Dot blot
Use at an assay dependent dilution. |
靶标
-
相关性
A hallmark of signal transduction pathways is the reversible phosphorylation of serine and threonine residues within specific sequences, or motifs, in target proteins. Specific signaling motifs include not only sequences that are recognized by protein kinases, but also those that are recognized by phosphorylation-dependent binding proteins like 14-3-3. These modular phosphoprotein interacting domains are critical elements in modulating, directing and amplifying intracellular communications. Many critical protein kinases can be regulated by phosphorylation at a specific serine or threonine surrounded by phenylalanine or tyrosine. For example, Akt, an important kinase that regulates cell survival, is activated by phosphorylation at Ser473, a site surrounded by phenylalanine and tyrosine. RSK1, p70 S6 K, and certain PKC isoforms also contain a similar consensus phosphorylation site. Phosphorylation of these sites is required for kinase activity. -
别名
- Phosphoserine antibody
- Phosphothreonine antibody
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (13)
ab117253 被引用在 13 文献中.
- Shen Y et al. Methionine oxidation of CLK4 promotes the metabolic switch and redox homeostasis in esophageal carcinoma via inhibiting MITF selective autophagy. Clin Transl Med 12:e719 (2022). PubMed: 35092699
- Wang M et al. Blockade of phosphotyrosine pathways suggesting SH2 superbinder as a novel therapy for pulmonary fibrosis. Theranostics 12:4513-4535 (2022). PubMed: 35832075
- Wang Z et al. GlPP2C1 Silencing Increases the Content of Ganodermalingzhi Polysaccharide (GL-PS) and Enhances Slt2 Phosphorylation. J Fungi (Basel) 8:N/A (2022). PubMed: 36135674
- Lan C et al. Inhibition of DYRK1A, via histone modification, promotes cardiomyocyte cell cycle activation and cardiac repair after myocardial infarction. EBioMedicine 82:104139 (2022). IP ; Rat . PubMed: 35810562
- Xia C et al. Phosphatase OsPP2C27 directly dephosphorylates OsMAPK3 and OsbHLH002 to negatively regulate cold tolerance in rice. Plant Cell Environ 44:491-505 (2021). PubMed: 33150964