Anti-Phosphoserine抗体[PSR-45] (ab6639)


  • 产品名称Anti-Phosphoserine抗体[PSR-45]
    参阅全部 Phosphoserine 一抗
  • 描述
    小鼠单克隆抗体[PSR-45] to Phosphoserine
  • 特异性Monoclonal Anti-Phosphoserine reacts against phosphorylated serine both as free amino acid or when conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP.
  • 经测试应用适用于: Dot blot, IP, Indirect ELISA, WBmore details
  • 种属反应性
    与反应: Species independent
  • 免疫原

    Other Immunogen Type corresponding to Phosphoserine conjugated to keyhole limpet haemocyanin.


    (Peptide available as ab100812)

  • 阳性对照
    • WB: rat brain cortex lysate ( a main 50kDa band is seen by detection with biotin-avidin AEC staining) ELISA: phosphoserine conjugated to BSA
  • 常规说明

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.



Our Abpromise guarantee covers the use of ab6639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot Use at an assay dependent concentration.
IP 1/2000.
Indirect ELISA 1/4000.
WB 1/500 - 1/1000. Can be blocked with Synthetic Phosphoserine protein (BSA) (ab100812). Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead. Stronger blocking agent percentage is recommended (e.g. 10%), in addition to varying loading amounts and shorter incubation time (1-2h RT)


  • 相关性Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
  • 别名
    • phospho-Ser antibody
    • pS antibody
    • pSER antibody

Anti-Phosphoserine antibody [PSR-45] 图像

  • Anti-Phosphoserine antibody [PSR-45] (ab6639) at 1/200 dilution + Drosophila melanogaster whole tissue lysate 40 ug per lane at 40 µg

    Sheep anti mouse (HRP) at 1/2000 dilution

    Performed under reducing conditions.

    See Abreview

  • ab6639 used in Immunoprecipitation.
    Whole cell lysate prepared from murine mammary cells was loaded at 250µg.
    Cells were treated with 5ng/ml TGF beta for varying time periods.
    Protein A was used for the Immunoprecipitation step.
    ab6639 used at a 1/2000 dilution for 10 hours at 4°C.
    Western blot was performed with an HRP anti-PCBP1 polyclonal antibody, 1/10000 dilution.

    See Abreview

Anti-Phosphoserine antibody [PSR-45] (ab6639)参考文献

This product has been referenced in:
  • Kusumoto-Matsuo R  et al. Serines 440 and 467 in the Werner syndrome protein are phosphorylated by DNA-PK and affects its dynamics in response to DNA double strand breaks. Aging (Albany NY) 6:70-81 (2014). WB . Read more (PubMed: 24429382) »
  • Cho KI  et al. Differential loss of prolyl isomerase or chaperone activity of Ran-binding protein 2 (Ranbp2) unveils distinct physiological roles of its cyclophilin domain in proteostasis. J Biol Chem 289:4600-25 (2014). WB . Read more (PubMed: 24403063) »

See all 13 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (NSCLC)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 30 µg
Treatment 0-100 nM volasertib for 2 Hrs
Specification NSCLC
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Mar 11 2017

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Yes, the use of PBS is a good point. In case of using anti-phospho antibodies this can actually cause high background due to the presence of phosphate in the buffer. I would sugg...

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I am sorry to hear that the 2 antibodies have not been working as expected.

Your protocol looks fine in general. However, both antibodies are directed against ANY phospho-site on a serine or threonine o...

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I am sorry to confirm that ab6639 Phosphoserine antibody [PSR-45] has only been tested in rat brain tissue bythe originator, and so we have no images for human samples.

There have, h...

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To our knowledge this product has not been tested in IHC. For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
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