Anti-Phosphoserine抗体(Biotin) (ab9335)

概述

  • 产品名称Anti-Phosphoserine抗体(Biotin)
    参阅全部 Phosphoserine 一抗
  • 描述
    兔多克隆抗体to Phosphoserine (Biotin)
  • 偶联物Biotin
  • 特异性Specifically recognizes both free-phosphoserine, serine-phosphorylated peptide and proteins. Does not cross-react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Slight cross-reactivity with free phosphothreonine. Readily reacts to known phosphoproteins such as phosvitin and alpha casein.
  • 经测试应用适用于: WB, IP, ELISAmore details
  • 种属反应性
    与反应: Species independent
  • 免疫原

    BSA and KLH-phosphoserine conjugates.

  • 阳性对照
    • Use Mouse brain extract for Western Blotting or synthetic phosphopeptide or phosvitin for ELISA.

性能

应用

Our Abpromise guarantee covers the use of ab9335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 4 µg/ml. Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead.
IP Use at an assay dependent dilution.
ELISA Use a concentration of 0.5 µg/ml.

靶标

  • 相关性Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
  • 别名
    • phospho-Ser antibody
    • pS antibody
    • pSER antibody

Anti-Phosphoserine antibody (Biotin) (ab9335)参考文献

This product has been referenced in:
  • Phillips JE  et al. Glucocorticoid-induced osteogenesis is negatively regulated by Runx2/Cbfa1 serine phosphorylation. J Cell Sci 119:581-91 (2006). WB . Read more (PubMed: 16443755) »

See 1 Publication for this product

Product Wall

All the information that we have regarding ab9335 is located on our online datasheet. Ab9335 specifically recognizes both free-phosphoserine, serine-phosphorylated peptide and proteins, and for a positive control it is recommended to use mouse brain ex...

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Thank you for your enquiry. The anti-phosphoserine antibodies will not cross-react with phosphotidylserine and other phospholipids.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"