Anti-Phosphoserine抗体[B106.1] (ab7851)

概述

  • 产品名称Anti-Phosphoserine抗体[B106.1]
    参阅全部 Phosphoserine 一抗
  • 描述
    小鼠单克隆抗体[B106.1] to Phosphoserine
  • 特异性This antibody detects free and conjugated phosphoserine as well as phosphoserine in modified cellular proteins. It does not cross react with nonphosphorylated serine or with phosphotyrosine or phosphothreonine, neither does it cross react with AMP or ATP. However, due to steric hinderance, certain proteins known to contain phosphorylated serine may not be recognized by this antibody.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Species independent
  • 免疫原

    Chemical/ Small Molecule corresponding to Phosphoserine conjugated to Keyhole Limpet Haemocyanin (KLH).

  • 阳性对照
    • For immunoblotting we recommend extract of rat brain cortex.

性能

应用

Our Abpromise guarantee covers the use of ab7851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50. Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead.

靶标

  • 相关性Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
  • 别名
    • phospho-Ser antibody
    • pS antibody
    • pSER antibody

Anti-Phosphoserine antibody [B106.1] (ab7851)参考文献

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See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (MIN6, Pancreatic B-cell line)
Specification MIN6, Pancreatic B-cell line
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%
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提交于 Sep 12 2005

Thank you for your enquiry. This antibody reacts with human. Other species have not been tested, however, since this antibody is directed toward phosphorylated serine, we expect it to cross-react with all animal species containing phosphoserine. ...

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This antibody has never been used in IP, therefore we can't guarantee results.

There may be phosphoserines present that are not detected. Due to steric hindrance, certain proteins known to contain phosphorylated serine may not be recognized by this antibody. (this extract from the datasheet previously read as "threonine" in pla...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"