Anti-Phosphoribosyl pyrophosphate amidotransferase抗体(ab125864)
Key features and details
- Rabbit polyclonal to Phosphoribosyl pyrophosphate amidotransferase
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Phosphoribosyl pyrophosphate amidotransferase抗体
参阅全部 Phosphoribosyl pyrophosphate amidotransferase 一抗 -
描述
兔多克隆抗体to Phosphoribosyl pyrophosphate amidotransferase -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 283-467 of Human Phosphoribosyl pyrophosphate amidotransferase (NP_002694).
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阳性对照
- WB: HepG2 and HCT116 whole cell lysates. IF: HeLa whole cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125864于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 57 kDa.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 57 kDa. |
ICC/IF
1/100 - 1/1000. |
靶标
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组织特异性
Ubiquitously expressed. -
通路
Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2. -
序列相似性
In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.
Contains 1 glutamine amidotransferase type-2 domain. - Information by UniProt
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数据库链接
- Entrez Gene: 5471 Human
- Omim: 172450 Human
- SwissProt: Q06203 Human
- Unigene: 331420 Human
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别名
- Amidophosphoribosyltransferase [Precursor] antibody
- Amidophosphoribosyltransferase antibody
- ATase antibody
see all
图片
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All lanes : Anti-Phosphoribosyl pyrophosphate amidotransferase antibody (ab125864) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 57 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate labeling Phosphoribosyl pyrophosphate amidotransferase with ab125864 at 1/500 dilution. Costained with Hoechst 33342 (Bottom panel).
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab125864 被引用在 1 文献中.
- Hu X et al. Identification and Validation of Novel Biomarkers for Diagnosis and Prognosis of Hepatocellular Carcinoma. Front Oncol 10:541479 (2020). PubMed: 33102213