Anti-PLD2 (phospho Y169)抗体(ab78907)
Key features and details
- Rabbit polyclonal to PLD2 (phospho Y169)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PLD2 (phospho Y169)抗体
参阅全部 PLD2 一抗 -
描述
兔多克隆抗体to PLD2 (phospho Y169) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human PLD2 (phospho Y169).
Database link: NP_002654 -
阳性对照
- IHC-P: human brain tissue WB: extracts from Jurkat cells treated with TNF (20 ng/ml, 30 min)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab78907 was purified by affinity chromatography using an epitope specific phosphopeptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab78907于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 106 kDa.
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IHC-P |
1/50 - 1/100.
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说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 106 kDa. |
IHC-P
1/50 - 1/100. |
靶标
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功能
May have a role in signal-induced cytoskeletal regulation and/or endocytosis. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the phospholipase D family.
Contains 1 PH domain.
Contains 2 PLD phosphodiesterase domains.
Contains 1 PX (phox homology) domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5338 Human
- Entrez Gene: 18806 Mouse
- Entrez Gene: 25097 Rat
- Omim: 602384 Human
- SwissProt: O14939 Human
- SwissProt: P97813 Mouse
- SwissProt: P70498 Rat
- Unigene: 104519 Human
see all -
别名
- Choline phosphatase 2 antibody
- EC 3.1.4.4 antibody
- hPLD 2 antibody
see all
图片
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All lanes : Anti-PLD2 (phospho Y169) antibody (ab78907) at 1/500 dilution
Lane 1 : extracts from Jurkat cells, treated with TNF (20 ng/ml, 30 min)
Lane 2 : extracts from Jurkat cells, treated with TNF (20 ng/ml, 30 min) with immunizing peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 106 kDa
Observed band size: 94 kDa why is the actual band size different from the predicted?
Additional bands at: 140 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemical analysis of PLD2 in paraffin embedded human brain tissue using ab78907, at 1/50 dilution, in the presence (right image) and absence (left image) of the immunizing peptide.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab78907 被引用在 1 文献中.
- Xuan Y et al. Inhibition of chaperone-mediated autophagy reduces tumor growth and metastasis and promotes drug sensitivity in colorectal cancer. Mol Med Rep 23:N/A (2021). PubMed: 33760140