RabMAb

Anti-Phospholipase D1抗体[EP1506Y] (ab68150)

概述

  • 产品名称Anti-Phospholipase D1抗体[EP1506Y]
    参阅全部 Phospholipase D1 一抗
  • 描述
    兔单克隆抗体[EP1506Y] to Phospholipase D1
  • 经测试应用适用于: WB, ICC, Flow Cytmore details
    不适用于: IHC-P or IP
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A synthetic peptide corresponding to residues on the N terminus of human Phospholipase D1.

  • 阳性对照
    • Hela cell lysate
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab68150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).
ICC 1/100 - 1/250.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明Is unsuitable for IHC-P or IP.
  • 靶标

    • 功能Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic.
    • 组织特异性Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.
    • 序列相似性Belongs to the phospholipase D family.
      Contains 1 PH domain.
      Contains 2 PLD phosphodiesterase domains.
      Contains 1 PX (phox homology) domain.
    • 细胞定位Cytoplasm > perinuclear region. Endoplasmic reticulum membrane. Golgi apparatus membrane. Late endosome membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Choline phosphatase 1 antibody
      • hPLD1 antibody
      • Phosphatidylcholine-hydrolyzing phospholipase D1 antibody
      • Phospholipase D1 antibody
      • PLD 1 antibody
      • PLD1 antibody
      • PLD1_HUMAN antibody
      see all

    Anti-Phospholipase D1 antibody [EP1506Y] 图像

    • Anti-Phospholipase D1 antibody [EP1506Y] (ab68150) at 1/500 dilution + Hela cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 120 kDa
      Observed band size : 120 kDa

    Anti-Phospholipase D1 antibody [EP1506Y] (ab68150)参考文献

    This product has been referenced in:
    • Sulzmaier FJ  et al. PEA-15 potentiates H-Ras-mediated epithelial cell transformation through phospholipase D. Oncogene 31:3547-60 (2012). Read more (PubMed: 22105357) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"