重组Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783)抗体[EP1898Y] (ab76031)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1898Y] to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783)
- Suitable for: WB, ICC/IF, Dot blot
- Reacts with: Mouse, Human
概述
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产品名称
Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783)抗体[EP1898Y]
参阅全部 Phospholipase C gamma 1/PLC-gamma-1 一抗 -
描述
兔单克隆抗体[EP1898Y] to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, Dot blotmore details
不适用于: Flow Cyt,IHC-P or IP -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Jurkat cell lysates treated with pervanadate. ICC/IF: Jurkat cells treated with pervanadate.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1898Y -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76031于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/100000 - 1/200000. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).
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ICC/IF |
1/100.
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Dot blot |
1/1000.
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说明 |
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WB
1/100000 - 1/200000. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa). |
ICC/IF
1/100. |
Dot blot
1/1000. |
靶标
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功能
Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. -
序列相似性
Contains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 2 PH domains.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
结构域
The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1. -
翻译后修饰
The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
Ubiquitinated by CBLB in activated T-cells. -
细胞定位
Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment. - Information by UniProt
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数据库链接
- Entrez Gene: 5335 Human
- Entrez Gene: 18803 Mouse
- Omim: 172420 Human
- SwissProt: P19174 Human
- SwissProt: Q62077 Mouse
- Unigene: 268177 Human
- Unigene: 44463 Mouse
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别名
- 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
- 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
see all
图片
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All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : Jurkat treated with 50mM pervanadate for 5 minutes whole cell lysate
Lane 3 : Jurkat treated with 50mM pervanadate for 5 min whole cell lysate. Then the membrane was incubated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 149 kDa
Observed band size: 149 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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Immunocytochemistry/Immunofluorescence analysis of Jurkat +/- pervanadate (1mM, 30min) and Jurkat + pervanadate (1mM, 30min) + LP labelling Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) with ab76031 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. ab150077 (goat anti-rabbit IgG Alexa Fluor® 488) (1/1000) was used as the secondary antibody. The cells were co-stained with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at a 1/200 dilution. Nuclei counterstained with DAPI (blue). Control: PBS instead of the primary antibody.
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All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 2 : NIH/3T3 treated with 50ng/ml PDGF-BB for 30 minutes whole cell lysate
Lane 3 : NIH/3T3 treated with 50ng/ml PDGF-BB for 30 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 149 kDa
Observed band size: 149 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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Dot blot analysis of Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) phospho peptide (Lane 1) and Phospholipase C gamma 1/PLC-gamma-1 non-phospho peptide (Lane 2) labelling Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) with ab76031 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.
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All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031) at 1/200000 dilution
Lane 1 : Jurkat cell lysates, untreated
Lane 2 : Jurkat cell lysates, treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 149 kDa
Observed band size: 149 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab76031 被引用在 2 文献中.
- Ma ZG et al. Toll-like receptor 5 deficiency diminishes doxorubicin-induced acute cardiotoxicity in mice. Theranostics 10:11013-11025 (2020). PubMed: 33042267
- Guedan S et al. Single residue in CD28-costimulated CAR-T cells limits long-term persistence and antitumor durability. J Clin Invest 130:3087-3097 (2020). PubMed: 32069268