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Anti-Phospholipase C gamma 1抗体[M156] (ab41433)

概述

  • 产品名称Anti-Phospholipase C gamma 1抗体[M156]
    参阅全部 Phospholipase C gamma 1 一抗
  • 描述
    小鼠单克隆抗体[M156] to Phospholipase C gamma 1
  • 特异性Phospholipase C gamma 1 immunising peptide sequence has low homology to Phospholipase C gamma 2.
  • 经测试应用适用于: Flow Cyt, WB, ELISA, IP, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to amino acids in the N-terminal region of human Phospholipase C gamma 1.

  • 阳性对照
    • Human A431, Hct116, and Jurkat cells; mouse brain.
  • 常规说明Do not aliquot.

性能

应用

Our Abpromise guarantee covers the use of ab41433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
ELISA 1/2000.
IP Use at an assay dependent concentration. 5-10ul of antibody per 300-500 ug of total protein lysate.
ICC/IF 1/100.

靶标

  • 功能Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
  • 序列相似性Contains 1 C2 domain.
    Contains 1 EF-hand domain.
    Contains 2 PH domains.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • 结构域The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
  • 翻译后修饰The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
    Ubiquitinated by CBLB in activated T-cells.
  • 细胞定位Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
    • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
    • 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
    • Inositoltrisphosphohydrolase antibody
    • Monophosphatidylinositol phosphodiesterase antibody
    • NCKAP3 antibody
    • Phosphatidylinositol phospholipase C antibody
    • Phosphoinositidase C antibody
    • Phosphoinositide phospholipase C antibody
    • Phosphoinositide phospholipase C-gamma-1 antibody
    • Phospholipase C 148 antibody
    • Phospholipase C gamma 1 antibody
    • Phospholipase C-gamma-1 antibody
    • Phospholipase C-II antibody
    • PLC gamma 1 antibody
    • PLC II antibody
    • PLC-148 antibody
    • PLC-gamma-1 antibody
    • PLC-II antibody
    • PLC1 antibody
    • PLC148 antibody
    • Plcg1 antibody
    • PLCG1_HUMAN antibody
    • PLCgamma1 antibody
    see all

Anti-Phospholipase C gamma 1 antibody [M156] 图像



  • Predicted band size : 150 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Phospholipase C gamma 1 knockout HAP1 cell lysate (20 µg)
    Lane 3: HepG2 cell lysate (20 µg)
    Lane 4: Mouse brain tissue lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab41433 observed at 160 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab41433 was shown to recognize Phospholipase C gamma 1 when Phospholipase C gamma 1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Phospholipase C gamma 1 knockout samples were subjected to SDS-PAGE. ab41433 diluted to 1/1000 and ab181602 (loading control to GAPDH) diluted to 1/10,000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.



  • Predicted band size : 150 kDa
    Western blot analysis of PLC gamma 1 immunoprecipitates from human jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2, 4, 5 & 6). Immunoprecipitation was performed with monoclonal anti-PLC gamma 1 (ab41433). The blots were probed with anti- PLC gamma 1 (lanes 1 & 2) and anti-PLC gamma 1 (Tyr-775) (lanes 3-6). The latter antibody was used in the presence of phospho- PLC gamma 1 (Tyr-775) peptide (lane 5), or a non-specific phosphotyrosine peptide (lane 6).
  • Overlay histogram showing Jurkat cells stained with ab41433 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab41433, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Phospholipase C gamma 1 antibody [M156] (ab41433)参考文献

This product has been referenced in:
  • Mizrachy-Schwartz S  et al. Up-regulation of AMP-activated protein kinase in cancer cell lines is mediated through c-Src activation. J Biol Chem 286:15268-77 (2011). WB ; Human . Read more (PubMed: 21245141) »

See 1 Publication for this product

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ELISA

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Cell (Jurkat Cells)
Specification Jurkat Cells
Type Sandwich (Capture)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jun 06 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (S2 cells line)
Loading amount 1e+006 cells
Specification S2 cells line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 21 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 40 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Mr. Jörg Hoehfeld

Verified customer

提交于 Jun 27 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"