Recombinant full length protein corresponding to Human Phospholipase C gamma 1. Database link: P19174
HepG2 whole cell lysate.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab76155 as a replacement.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
1/2000. Predicted molecular weight: 149 kDa.
Use a concentration of 1 µg/ml.
Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ. Ubiquitinated by CBLB in activated T-cells.
Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
Western blot - Anti-Phospholipase C gamma 1 antibody [2B1] (ab16955)
Predicted band size : 149 kDa
Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: Phospholipase C gamma 1 knockout HAP1 cell lysate (20 µg) Lane 3: HepG2 cell lysate (20 µg) Lane 4: Mouse brain tissue lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab16955 observed at 160 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab16955 was shown to recognize Phospholipase C gamma 1 when Phospholipase C gamma 1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Phospholipase C gamma 1 knockout samples were subjected to SDS-PAGE. ab16955 at a dilution of 1/2000 and ab181602 (loading control to GAPDH) at a dilution of 1/10,000 were incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Western blot - Phospholipase C gamma antibody [2B1] (ab16955)
All lanes : Anti-Phospholipase C gamma 1 antibody [2B1] (ab16955)
Lane 1 : HepG2 cell lysates Lane 2 : 293T cell lysates Lane 3 : Mouse brain
Flow Cytometry-Anti-Phospholipase C gamma 1 antibody [2B1](ab16955)
Overlay histogram showing Jurkat cells stained with ab16955 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16955, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Gao G et al. Periodic mechanical stress induces the extracellular matrix expression and migration of rat nucleus pulposus cells by upregulating the expression of intergrin a1 and phosphorylation of downstream phospholipase C?1. Mol Med Rep14:2457-64 (2016).
Read more (PubMed: 27484337) »
Verhaar AP et al. Superantigen-induced steroid resistance depends on activation of phospholipase Cß2. J Immunol190:6589-95 (2013).
Read more (PubMed: 23690479) »