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Anti-Phospholipase C gamma 1抗体[2B1] (ab16955)

概述

  • 产品名称Anti-Phospholipase C gamma 1抗体[2B1]
    参阅全部 Phospholipase C gamma 1 一抗
  • 描述
    小鼠单克隆抗体[2B1] to Phospholipase C gamma 1
  • 经测试应用适用于: Flow Cyt, ELISA, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Ferret
  • 免疫原

    Recombinant full length protein corresponding to Human Phospholipase C gamma 1.
    Database link: P19174

  • 阳性对照
    • HepG2 whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab16955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.
WB 1/2000. Predicted molecular weight: 149 kDa.
IP Use a concentration of 1 µg/ml.

靶标

  • 功能Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
  • 序列相似性Contains 1 C2 domain.
    Contains 1 EF-hand domain.
    Contains 2 PH domains.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • 结构域The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
  • 翻译后修饰The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
    Ubiquitinated by CBLB in activated T-cells.
  • 细胞定位Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
    • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
    • 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
    • Inositoltrisphosphohydrolase antibody
    • Monophosphatidylinositol phosphodiesterase antibody
    • NCKAP3 antibody
    • Phosphatidylinositol phospholipase C antibody
    • Phosphoinositidase C antibody
    • Phosphoinositide phospholipase C antibody
    • Phosphoinositide phospholipase C-gamma-1 antibody
    • Phospholipase C 148 antibody
    • Phospholipase C gamma 1 antibody
    • Phospholipase C-gamma-1 antibody
    • Phospholipase C-II antibody
    • PLC gamma 1 antibody
    • PLC II antibody
    • PLC-148 antibody
    • PLC-gamma-1 antibody
    • PLC-II antibody
    • PLC1 antibody
    • PLC148 antibody
    • Plcg1 antibody
    • PLCG1_HUMAN antibody
    • PLCgamma1 antibody
    see all

Anti-Phospholipase C gamma 1 antibody [2B1] 图像



  • Predicted band size : 149 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Phospholipase C gamma 1 knockout HAP1 cell lysate (20 µg)
    Lane 3: HepG2 cell lysate (20 µg)
    Lane 4: Mouse brain tissue lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab16955 observed at 160 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab16955 was shown to recognize Phospholipase C gamma 1 when Phospholipase C gamma 1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Phospholipase C gamma 1 knockout samples were subjected to SDS-PAGE. ab16955 at a dilution of 1/2000 and ab181602 (loading control to GAPDH) at a dilution of 1/10,000 were incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-Phospholipase C gamma 1 antibody [2B1] (ab16955)

    Lane 1 : HepG2 cell lysates
    Lane 2 : 293T cell lysates
    Lane 3 : Mouse brain


    Predicted band size : 149 kDa
    Observed band size : 175 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing Jurkat cells stained with ab16955 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16955, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Phospholipase C gamma 1 antibody [2B1] (ab16955)参考文献

This product has been referenced in:
  • Verhaar AP  et al. Superantigen-induced steroid resistance depends on activation of phospholipase Cß2. J Immunol 190:6589-95 (2013). Human . Read more (PubMed: 23690479) »
  • Xu R  et al. Oxidative stress mediates the disruption of airway epithelial tight junctions through a TRPM2-PLC?1-PKCa signaling pathway. Int J Mol Sci 14:9475-86 (2013). WB ; Human . Read more (PubMed: 23629676) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (whole cells - S2-DRSC)
Loading amount 1e+006 cells
Specification whole cells - S2-DRSC
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 21 2009

Thank you for your enquiry and your interest in our products. I would like to confirm that ab16955 only detects PLCg1 and this antibody has been tested and characterised for Western blot application. My colleagues will add the Western blot image to ...

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